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Safe cosmetics without animal testing? Contributions of the EU Project Sens-it-iv

Weltzien, Hans Ulrich; Corsini, Emanuela; Gibbs, Sue; Lindstedt, Malin LU ; Borrebaeck, Carl LU ; Budde, Petra; Schulz-Knappe, Peter; Thierse, Hermann-Josef; Martin, Stefan F. and Roggen, Erwin L. (2009) In Journal für Verbraucherschütz und Lebensmittelsicherheit 4(suppl. 2). p.41-48
Abstract
The 7th Ammendment to the Cosmetics Directive of the European Commission (Directive 76/768/EEC2) bans the marketing of cosmetics containing animal-tested ingredients since March 2009. Excepted are only tests for repeated dose toxicity, for which the animal ban will come into effect by 2013. One major concern for cosmetics, i.e. the risk of containing skin (contact) sensitizers, has in the past been addressed almost exclusively by animal testing. It is this problem attracting the central interest of the integrated research project Sens-it-iv (Novel Testing Strategies for in vitro Assessment of Allergens, http://www.sens-it-iv.eu ), funded by the EC within framework 6 since October 2005. Here, the 28 Sens-it-iv partners from 10 European... (More)
The 7th Ammendment to the Cosmetics Directive of the European Commission (Directive 76/768/EEC2) bans the marketing of cosmetics containing animal-tested ingredients since March 2009. Excepted are only tests for repeated dose toxicity, for which the animal ban will come into effect by 2013. One major concern for cosmetics, i.e. the risk of containing skin (contact) sensitizers, has in the past been addressed almost exclusively by animal testing. It is this problem attracting the central interest of the integrated research project Sens-it-iv (Novel Testing Strategies for in vitro Assessment of Allergens, http://www.sens-it-iv.eu ), funded by the EC within framework 6 since October 2005. Here, the 28 Sens-it-iv partners from 10 European States present the 5 most promising types of in vitro assays selected for further refinement. These are: (1) a human epidermal equivalent (EE) model to rank contact allergens according to their sensitizing potency, (2) identification of contact sensitizers, including pro-haptens, through intracellular production of IL-18 by the human keratinocyte cell line NCTC 2544, (3) determination of activation markers such as CD86, CD54 and most prominently CXCL8 (IL-8) on/in dendritic cell lines, (4) contact sensitizer-specific migration of MUTZ Langerhans cells towards the chemokine CXCL12, and (5) the allergen-specific activation and proliferation of na < ve human T cells. Ongoing genomic and proteomic experiments are in the process of identifying larger sensitizer-specific biological marker signatures to be integrated into the above assays. We hope to supply the European control agencies with a basis for further validation of in vitro assays by the end of 2010. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Allergy, Contact, Sensitizer, In vitro, Alternative, Animal, Cosmetic
in
Journal für Verbraucherschütz und Lebensmittelsicherheit
volume
4
issue
suppl. 2
pages
41 - 48
publisher
Birkhaüser
external identifiers
  • wos:000273787600009
  • scopus:78651301963
ISSN
1661-5867
DOI
10.1007/s00003-009-0510-5
language
English
LU publication?
yes
id
ebe38070-407c-4bbe-8e1c-9d628feb6eaf (old id 1546491)
date added to LUP
2010-02-26 09:27:12
date last changed
2017-12-10 03:42:07
@article{ebe38070-407c-4bbe-8e1c-9d628feb6eaf,
  abstract     = {The 7th Ammendment to the Cosmetics Directive of the European Commission (Directive 76/768/EEC2) bans the marketing of cosmetics containing animal-tested ingredients since March 2009. Excepted are only tests for repeated dose toxicity, for which the animal ban will come into effect by 2013. One major concern for cosmetics, i.e. the risk of containing skin (contact) sensitizers, has in the past been addressed almost exclusively by animal testing. It is this problem attracting the central interest of the integrated research project Sens-it-iv (Novel Testing Strategies for in vitro Assessment of Allergens, http://www.sens-it-iv.eu ), funded by the EC within framework 6 since October 2005. Here, the 28 Sens-it-iv partners from 10 European States present the 5 most promising types of in vitro assays selected for further refinement. These are: (1) a human epidermal equivalent (EE) model to rank contact allergens according to their sensitizing potency, (2) identification of contact sensitizers, including pro-haptens, through intracellular production of IL-18 by the human keratinocyte cell line NCTC 2544, (3) determination of activation markers such as CD86, CD54 and most prominently CXCL8 (IL-8) on/in dendritic cell lines, (4) contact sensitizer-specific migration of MUTZ Langerhans cells towards the chemokine CXCL12, and (5) the allergen-specific activation and proliferation of na &lt; ve human T cells. Ongoing genomic and proteomic experiments are in the process of identifying larger sensitizer-specific biological marker signatures to be integrated into the above assays. We hope to supply the European control agencies with a basis for further validation of in vitro assays by the end of 2010.},
  author       = {Weltzien, Hans Ulrich and Corsini, Emanuela and Gibbs, Sue and Lindstedt, Malin and Borrebaeck, Carl and Budde, Petra and Schulz-Knappe, Peter and Thierse, Hermann-Josef and Martin, Stefan F. and Roggen, Erwin L.},
  issn         = {1661-5867},
  keyword      = {Allergy,Contact,Sensitizer,In vitro,Alternative,Animal,Cosmetic},
  language     = {eng},
  number       = {suppl. 2},
  pages        = {41--48},
  publisher    = {Birkhaüser},
  series       = {Journal für Verbraucherschütz und Lebensmittelsicherheit},
  title        = {Safe cosmetics without animal testing? Contributions of the EU Project Sens-it-iv},
  url          = {http://dx.doi.org/10.1007/s00003-009-0510-5},
  volume       = {4},
  year         = {2009},
}