Advanced

Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects

Quillaguaman, Jorge; Guzmán, Hector LU ; Doan Van, Thuoc LU and Hatti-Kaul, Rajni LU (2010) In Applied Microbiology and Biotechnology 85(6). p.1687-1696
Abstract
Biodegradable materials with plastic or elastomeric properties are in great demand for a variety of applications. Polyhydroxyalkanoates (PHAs), polyesters synthesized by microorganisms, possess such desired features. Industrial production of PHAs is currently achieved using recombinant Escherichia coli. Nevertheless, recent research on halophiles, salt requiring microorganisms, has shown a remarkable potential for biotechnological production of PHAs. The halophilic archaeon Haloferax mediterranei accumulates a co-polymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in large amounts using glucose, starch, and hydrolyzed whey as carbon sources. Chemical composition and molecular weight of PHAs produced by H. mediterranei can be... (More)
Biodegradable materials with plastic or elastomeric properties are in great demand for a variety of applications. Polyhydroxyalkanoates (PHAs), polyesters synthesized by microorganisms, possess such desired features. Industrial production of PHAs is currently achieved using recombinant Escherichia coli. Nevertheless, recent research on halophiles, salt requiring microorganisms, has shown a remarkable potential for biotechnological production of PHAs. The halophilic archaeon Haloferax mediterranei accumulates a co-polymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in large amounts using glucose, starch, and hydrolyzed whey as carbon sources. Chemical composition and molecular weight of PHAs produced by H. mediterranei can be modified depending on the substrate utilized as precursor. Phylogenetic studies on haloarchaeal enzymes able to polymerize the components of PHAs (i.e., PHA synthases) reveal a novel cluster, with a close relationship with PHA polymerases of bacteria and archaea found in marine-related niches. On the other hand, sequences of PHA synthases of two halophilic bacteria are more closely affiliated to synthases of Proteobacteria. Several bacterial species of the family Halomonadaceae accumulate PHAs. Halomonas boliviensis reached PHA yields and volumetric productivities close to the highest reported so far. Furthermore, H. boliviensis and other Halomonas species are able to co-produce PHA and osmolytes, i.e., ectoines and hydroxyectoine, in one process. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
boliviensis, Halomonas, Haloferax mediterranei, Polyhydroxyalkanoates, Halophiles, Osmolytes, Ectoines
in
Applied Microbiology and Biotechnology
volume
85
issue
6
pages
1687 - 1696
publisher
Springer
external identifiers
  • wos:000273978400006
  • scopus:76849099236
ISSN
1432-0614
DOI
10.1007/s00253-009-2397-6
language
English
LU publication?
yes
id
9aa0bbac-456c-4915-beed-0e994c2e71e4 (old id 1547048)
date added to LUP
2010-02-25 10:07:01
date last changed
2018-05-29 12:31:02
@article{9aa0bbac-456c-4915-beed-0e994c2e71e4,
  abstract     = {Biodegradable materials with plastic or elastomeric properties are in great demand for a variety of applications. Polyhydroxyalkanoates (PHAs), polyesters synthesized by microorganisms, possess such desired features. Industrial production of PHAs is currently achieved using recombinant Escherichia coli. Nevertheless, recent research on halophiles, salt requiring microorganisms, has shown a remarkable potential for biotechnological production of PHAs. The halophilic archaeon Haloferax mediterranei accumulates a co-polymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in large amounts using glucose, starch, and hydrolyzed whey as carbon sources. Chemical composition and molecular weight of PHAs produced by H. mediterranei can be modified depending on the substrate utilized as precursor. Phylogenetic studies on haloarchaeal enzymes able to polymerize the components of PHAs (i.e., PHA synthases) reveal a novel cluster, with a close relationship with PHA polymerases of bacteria and archaea found in marine-related niches. On the other hand, sequences of PHA synthases of two halophilic bacteria are more closely affiliated to synthases of Proteobacteria. Several bacterial species of the family Halomonadaceae accumulate PHAs. Halomonas boliviensis reached PHA yields and volumetric productivities close to the highest reported so far. Furthermore, H. boliviensis and other Halomonas species are able to co-produce PHA and osmolytes, i.e., ectoines and hydroxyectoine, in one process.},
  author       = {Quillaguaman, Jorge and Guzmán, Hector and Doan Van, Thuoc and Hatti-Kaul, Rajni},
  issn         = {1432-0614},
  keyword      = {boliviensis,Halomonas,Haloferax mediterranei,Polyhydroxyalkanoates,Halophiles,Osmolytes,Ectoines},
  language     = {eng},
  number       = {6},
  pages        = {1687--1696},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects},
  url          = {http://dx.doi.org/10.1007/s00253-009-2397-6},
  volume       = {85},
  year         = {2010},
}