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Helicobacter species DNA in liver and gastric tissues in children and adolescents with chronic liver disease

Casswall, Thomas H.; Nemeth, Antal; Nilsson, Ingrid LU ; Wadström, Torkel LU and Nilsson, Hans-Olof LU (2010) In Scandinavian Journal of Gastroenterology 45(2). p.160-167
Abstract
Objective. Enterohepatic Helicobacter species (EHS) have previously been found in adults with hepatobiliary diseases. Here, we report the prevalence of Helicobacter pylori and EHS in liver and gastric tissue in children and adolescents with chronic liver disease (CLD). Material and methods. Seventy-seven consecutive children and adolescents with CLD with or without ulcerative colitis or Crohn's disease (UC/CD) were investigated. Tissue samples were analysed using a Helicobacter genus specific 16S rDNA polymerase chain reaction (PCR) assay and DNA-sequence analysis. Sera from 61 subjects were also analysed using enzyme immunoassay and immunoblotting. Results. The Helicobacter PCR was positive in 3/23 (13%) livers from patients with primary... (More)
Objective. Enterohepatic Helicobacter species (EHS) have previously been found in adults with hepatobiliary diseases. Here, we report the prevalence of Helicobacter pylori and EHS in liver and gastric tissue in children and adolescents with chronic liver disease (CLD). Material and methods. Seventy-seven consecutive children and adolescents with CLD with or without ulcerative colitis or Crohn's disease (UC/CD) were investigated. Tissue samples were analysed using a Helicobacter genus specific 16S rDNA polymerase chain reaction (PCR) assay and DNA-sequence analysis. Sera from 61 subjects were also analysed using enzyme immunoassay and immunoblotting. Results. The Helicobacter PCR was positive in 3/23 (13%) livers from patients with primary sclerosing cholangitis and UC, and in 1/2 livers from patients with autoimmune hepatitis (AIH) and UC. Sequenced PCR products matched the 16S rDNA of H. hepaticus, H. muridarum, H. canis, and H. pylori, respectively. H. ganmani and H. bilis were detected in gastric tissues from two AIH patients. H. hepaticus and H. pullorum were found in livers from two patients with acute liver failure and intrahepatic cholestasis. Antibody reactivity to Helicobacter cell-surface proteins was negative. Conclusions. H. pylori and EHS can be detected in the livers of some patients with UC and concomitant liver disease, as well as in other children with liver diseases. Multicentre studies from different locations are needed to find out whether these bacteria play a pathogenetic role or whether their presence is an epiphenomenon. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
colitis, ulcerative, primary sclerosing cholangitis, polymerase chain reaction, liver tissue, inflammatory bowel disease, Helicobacter pylori, enterohepatic Helicobacter species, Autoimmune hepatitis, children
in
Scandinavian Journal of Gastroenterology
volume
45
issue
2
pages
160 - 167
publisher
Taylor & Francis
external identifiers
  • wos:000273874200005
  • scopus:76749132674
ISSN
1502-7708
DOI
10.3109/00365520903426915
language
English
LU publication?
yes
id
12116a92-78a9-4e79-b5b2-bdfd53bcd199 (old id 1547156)
date added to LUP
2010-02-24 11:25:37
date last changed
2018-05-29 10:39:54
@article{12116a92-78a9-4e79-b5b2-bdfd53bcd199,
  abstract     = {Objective. Enterohepatic Helicobacter species (EHS) have previously been found in adults with hepatobiliary diseases. Here, we report the prevalence of Helicobacter pylori and EHS in liver and gastric tissue in children and adolescents with chronic liver disease (CLD). Material and methods. Seventy-seven consecutive children and adolescents with CLD with or without ulcerative colitis or Crohn's disease (UC/CD) were investigated. Tissue samples were analysed using a Helicobacter genus specific 16S rDNA polymerase chain reaction (PCR) assay and DNA-sequence analysis. Sera from 61 subjects were also analysed using enzyme immunoassay and immunoblotting. Results. The Helicobacter PCR was positive in 3/23 (13%) livers from patients with primary sclerosing cholangitis and UC, and in 1/2 livers from patients with autoimmune hepatitis (AIH) and UC. Sequenced PCR products matched the 16S rDNA of H. hepaticus, H. muridarum, H. canis, and H. pylori, respectively. H. ganmani and H. bilis were detected in gastric tissues from two AIH patients. H. hepaticus and H. pullorum were found in livers from two patients with acute liver failure and intrahepatic cholestasis. Antibody reactivity to Helicobacter cell-surface proteins was negative. Conclusions. H. pylori and EHS can be detected in the livers of some patients with UC and concomitant liver disease, as well as in other children with liver diseases. Multicentre studies from different locations are needed to find out whether these bacteria play a pathogenetic role or whether their presence is an epiphenomenon.},
  author       = {Casswall, Thomas H. and Nemeth, Antal and Nilsson, Ingrid and Wadström, Torkel and Nilsson, Hans-Olof},
  issn         = {1502-7708},
  keyword      = {colitis,ulcerative,primary sclerosing cholangitis,polymerase chain reaction,liver tissue,inflammatory bowel disease,Helicobacter pylori,enterohepatic Helicobacter species,Autoimmune hepatitis,children},
  language     = {eng},
  number       = {2},
  pages        = {160--167},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Helicobacter species DNA in liver and gastric tissues in children and adolescents with chronic liver disease},
  url          = {http://dx.doi.org/10.3109/00365520903426915},
  volume       = {45},
  year         = {2010},
}