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Antioxidants rescue photoreceptors in rd1 mice: Relationship with thiol metabolism

Miranda, Maria; Arnal, Emma; Ahuja, Satpal LU ; Alvarez-Nolting, Raquel; Lopez-Pedrajas, Rosa; Ekström, Per LU ; Bosch-Morell, Francisco; van Veen, Theo LU and Romero, Francisco J. (2010) In Free Radical Biology & Medicine 48(2). p.216-222
Abstract
We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between... (More)
We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between glutathione retinal concentration and number of TUNEL-positive cells. No difference was observed between the numbers of nNOS- and NADPH-diaphorase-positive cells in treated and untreated rd1 mice. Thiol contents and thiol-dependent peroxide metabolism seem to be directly related to the survival of photoreceptors in rd1 mouse retina. (C) 2009 Elsevier Inc. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
radicals, Free, Nitric oxide, Glutathione, Antioxidants, Retina, Photoreceptors
in
Free Radical Biology & Medicine
volume
48
issue
2
pages
216 - 222
publisher
Elsevier
external identifiers
  • wos:000273883800005
  • scopus:72649084334
ISSN
0891-5849
DOI
10.1016/j.freeradbiomed.2009.10.042
language
English
LU publication?
yes
id
946d1648-d033-496b-af09-53f1c5742132 (old id 1547320)
date added to LUP
2010-02-24 09:36:05
date last changed
2018-05-29 10:14:27
@article{946d1648-d033-496b-af09-53f1c5742132,
  abstract     = {We have previously shown that the use of a combination of antioxidants delayed the degeneration process in rd1 mouse retina. In an effort to understand the mechanism of action of these substances (zeaxanthin, lutein, alpha-lipoic acid, glutathione, and Lycium barbarum extract) the changes in the levels of several proteins and oxidative stress markers in the rd1 retina have been studied. The treatment increased glutathione peroxidase activity and glutathione levels and decreased cystine concentrations in rd1 retinas. Considering all the results obtained from treated and untreated animals, a high correlation was present between glutathione concentration and glutathione peroxidase activity, and there was a negative correlation between glutathione retinal concentration and number of TUNEL-positive cells. No difference was observed between the numbers of nNOS- and NADPH-diaphorase-positive cells in treated and untreated rd1 mice. Thiol contents and thiol-dependent peroxide metabolism seem to be directly related to the survival of photoreceptors in rd1 mouse retina. (C) 2009 Elsevier Inc. All rights reserved.},
  author       = {Miranda, Maria and Arnal, Emma and Ahuja, Satpal and Alvarez-Nolting, Raquel and Lopez-Pedrajas, Rosa and Ekström, Per and Bosch-Morell, Francisco and van Veen, Theo and Romero, Francisco J.},
  issn         = {0891-5849},
  keyword      = {radicals,Free,Nitric oxide,Glutathione,Antioxidants,Retina,Photoreceptors},
  language     = {eng},
  number       = {2},
  pages        = {216--222},
  publisher    = {Elsevier},
  series       = {Free Radical Biology & Medicine},
  title        = {Antioxidants rescue photoreceptors in rd1 mice: Relationship with thiol metabolism},
  url          = {http://dx.doi.org/10.1016/j.freeradbiomed.2009.10.042},
  volume       = {48},
  year         = {2010},
}