CD4+T-cell localization to the large intestinal mucosa during Trichuris muris infection is mediated by G alpha(i)-coupled receptors but is CCR6-and CXCR3-independent
(2010) In Immunology 129(2). p.257-267- Abstract
- P>Infection of mice with the gastrointestinal nematode Trichuris muris represents a valuable tool to investigate and dissect intestinal immune responses. Resistant mouse strains respond to T. muris infection by mounting a T helper type 2 response. Previous results have shown that CD4+ T cells play a critical role in protective immunity, and that CD4+ T cells localize to the infected large intestinal mucosa to confer protection. Further, transfer of CD4+ T cells from immune mice to immunodeficient SCID mice can prevent the development of a chronic infection. In the current study, we characterize the protective CD4+ T cells, describe their chemokine receptor expression and explore the functional significance of these receptors in... (More)
- P>Infection of mice with the gastrointestinal nematode Trichuris muris represents a valuable tool to investigate and dissect intestinal immune responses. Resistant mouse strains respond to T. muris infection by mounting a T helper type 2 response. Previous results have shown that CD4+ T cells play a critical role in protective immunity, and that CD4+ T cells localize to the infected large intestinal mucosa to confer protection. Further, transfer of CD4+ T cells from immune mice to immunodeficient SCID mice can prevent the development of a chronic infection. In the current study, we characterize the protective CD4+ T cells, describe their chemokine receptor expression and explore the functional significance of these receptors in recruitment to the large intestinal mucosa post-T. muris infection. We show that the ability to mediate expulsion resides within a subpopulation of CD4+ T cells marked by down-regulation of CD62L. These cells can be isolated from intestine-draining mesenteric lymph nodes (MLN) from day 14 post-infection, but are rare or absent in MLN before this and in spleen at all times post-infection. Among CD4+ CD62Llow MLN cells, the two most abundantly expressed chemokine receptors were CCR6 and CXCR3. We demonstrate for the first time that CD4+ CD62Llow T-cell migration to the large intestinal mucosa is dependent on the family of G alpha(i)-coupled receptors, to which chemokine receptors belong. CCR6 and CXCR3 were however dispensable for this process because neutralization of CCR6 and CXCR3 did not prevent CD4+ CD62Llow cell migration to the large intestinal mucosa during T. muris infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1547841
- author
- Svensson Frej, Marcus LU ; Russell, Karen ; Mack, Matthias and Else, Kathryn J.
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- helminths, mucosal immunity, trafficking, receptors, chemokine, Th17), Th3, Th2, Th1, Th0, CD4, helper T cells (Th cells
- in
- Immunology
- volume
- 129
- issue
- 2
- pages
- 257 - 267
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000273458000011
- scopus:73949129043
- pmid:19824922
- ISSN
- 0019-2805
- DOI
- 10.1111/j.1365-2567.2009.03178.x
- language
- English
- LU publication?
- yes
- id
- 0beea8f3-3d86-4b58-a4c3-6acb0e1b4b39 (old id 1547841)
- date added to LUP
- 2016-04-01 10:34:47
- date last changed
- 2022-01-26 00:33:59
@article{0beea8f3-3d86-4b58-a4c3-6acb0e1b4b39,
abstract = {{P>Infection of mice with the gastrointestinal nematode Trichuris muris represents a valuable tool to investigate and dissect intestinal immune responses. Resistant mouse strains respond to T. muris infection by mounting a T helper type 2 response. Previous results have shown that CD4+ T cells play a critical role in protective immunity, and that CD4+ T cells localize to the infected large intestinal mucosa to confer protection. Further, transfer of CD4+ T cells from immune mice to immunodeficient SCID mice can prevent the development of a chronic infection. In the current study, we characterize the protective CD4+ T cells, describe their chemokine receptor expression and explore the functional significance of these receptors in recruitment to the large intestinal mucosa post-T. muris infection. We show that the ability to mediate expulsion resides within a subpopulation of CD4+ T cells marked by down-regulation of CD62L. These cells can be isolated from intestine-draining mesenteric lymph nodes (MLN) from day 14 post-infection, but are rare or absent in MLN before this and in spleen at all times post-infection. Among CD4+ CD62Llow MLN cells, the two most abundantly expressed chemokine receptors were CCR6 and CXCR3. We demonstrate for the first time that CD4+ CD62Llow T-cell migration to the large intestinal mucosa is dependent on the family of G alpha(i)-coupled receptors, to which chemokine receptors belong. CCR6 and CXCR3 were however dispensable for this process because neutralization of CCR6 and CXCR3 did not prevent CD4+ CD62Llow cell migration to the large intestinal mucosa during T. muris infection.}},
author = {{Svensson Frej, Marcus and Russell, Karen and Mack, Matthias and Else, Kathryn J.}},
issn = {{0019-2805}},
keywords = {{helminths; mucosal immunity; trafficking; receptors; chemokine; Th17); Th3; Th2; Th1; Th0; CD4; helper T cells (Th cells}},
language = {{eng}},
number = {{2}},
pages = {{257--267}},
publisher = {{Wiley-Blackwell}},
series = {{Immunology}},
title = {{CD4+T-cell localization to the large intestinal mucosa during Trichuris muris infection is mediated by G alpha(i)-coupled receptors but is CCR6-and CXCR3-independent}},
url = {{http://dx.doi.org/10.1111/j.1365-2567.2009.03178.x}},
doi = {{10.1111/j.1365-2567.2009.03178.x}},
volume = {{129}},
year = {{2010}},
}