Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.
Azadi, Seifollah LU ; Paquet-Durand, Francois LU ; Medstrand, Patrik LU
; van Veen, Theo
LU
and Ekström, Per
LU
(2006)
In Molecular and Cellular Neuroscience
31(4).
p.759-773
- Abstract
- The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation... (More)
- The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/154974
- author
- Azadi, Seifollah
LU
; Paquet-Durand, Francois
LU
; Medstrand, Patrik
LU
; van Veen, Theo
LU
and Ekström, Per
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Apoptosis, Kinase, Microarray, Photoreceptor, Signaling pathways
- in
- Molecular and Cellular Neuroscience
- volume
- 31
- issue
- 4
- pages
- 759 - 773
- publisher
- Elsevier
- external identifiers
-
- wos:000236659600015
- scopus:33645356186
- pmid:16503160
- ISSN
- 1044-7431
- DOI
- 10.1016/j.mcn.2006.01.001
- language
- English
- LU publication?
- yes
- id
- a0ed4b6d-4551-4c11-806e-66abb35d6e92 (old id 154974)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16503160&dopt=Abstract
- date added to LUP
- 2016-04-04 08:56:30
- date last changed
- 2022-03-31 00:43:09
@article{a0ed4b6d-4551-4c11-806e-66abb35d6e92,
abstract = {{The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.}},
author = {{Azadi, Seifollah and Paquet-Durand, Francois and Medstrand, Patrik and van Veen, Theo and Ekström, Per}},
issn = {{1044-7431}},
keywords = {{Apoptosis; Kinase; Microarray; Photoreceptor; Signaling pathways}},
language = {{eng}},
number = {{4}},
pages = {{759--773}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Neuroscience}},
title = {{Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.}},
url = {{http://dx.doi.org/10.1016/j.mcn.2006.01.001}},
doi = {{10.1016/j.mcn.2006.01.001}},
volume = {{31}},
year = {{2006}},
}