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Functional activity of insulinoma cells (INS-1E) and pancreatic islets cultured in agarose cryogel sponges

Bloch, K; Lozinsky, V I; Galaev, Igor LU ; Yavriyanz, K; Vorobeychik, M; Azarov, D; Damshkaln, L G; Mattiasson, Bo LU and Vardi, P (2005) In Journal of Biomedical Materials Research. Part A 75A(4). p.802-809
Abstract
Here, we describe the preparation, structure, and properties of cryogel sponges, which represent a new type of macroporous biomaterial for tissue engineering. Cryogels were produced through freeze-thawing techniques, either from agarose alone or from agarose with grafted gelatin. The aim of this study was to evaluate agarose cryogel sponges as scaffolds for Culturing both isolated pancreatic islets and insulinoma cells (INS-IE). In order to evaluate the effect of cell entrapment in artificial scaffolds, cell function reflected by insulin secretion and content was studied in cells cultivated for a 2-week period either in Culture plastic plates or in cryogel sponge disks. Our results show that tumor-derived INS-1E cells grown either on... (More)
Here, we describe the preparation, structure, and properties of cryogel sponges, which represent a new type of macroporous biomaterial for tissue engineering. Cryogels were produced through freeze-thawing techniques, either from agarose alone or from agarose with grafted gelatin. The aim of this study was to evaluate agarose cryogel sponges as scaffolds for Culturing both isolated pancreatic islets and insulinoma cells (INS-IE). In order to evaluate the effect of cell entrapment in artificial scaffolds, cell function reflected by insulin secretion and content was studied in cells cultivated for a 2-week period either in Culture plastic plates or in cryogel sponge disks. Our results show that tumor-derived INS-1E cells grown either on plastic or on cryogels do not differ in their proliferation, morphology, insulin release, and intracellular insulin content. However, isolated pancreatic islets cultivated on cryogels sponge show 15-fold higher basal insulin secretion at 3.0 mM glucose than islets cultivated on plastic plates and fail to respond to stimulation with 16.7 mM glucose. In addition, these islets have about 2-fold lower insulin content compared to those grown in plastic plates. It is possible that the cell dysfunction noted in these in vitro experiments is due to the effect of the limited oxygen supply to the islets cultivated in cryogel sponge. Further in vivo Studies are needed to clarify the nature of such an observation since according to previous reports, agarose and gelatin induce new vessel formation supporting enhanced oxygen supply. (c) 2005 Wiley Periodicals, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biomedical Materials Research. Part A
volume
75A
issue
4
pages
802 - 809
publisher
John Wiley & Sons
external identifiers
  • wos:000233690500006
  • pmid:16138321
  • scopus:28844490499
ISSN
1552-4965
DOI
10.1002/jbm.a.30466
language
English
LU publication?
yes
id
4cfb39a2-8e57-4915-a637-318644bd6139 (old id 154997)
date added to LUP
2007-07-02 08:24:29
date last changed
2017-11-19 03:27:29
@article{4cfb39a2-8e57-4915-a637-318644bd6139,
  abstract     = {Here, we describe the preparation, structure, and properties of cryogel sponges, which represent a new type of macroporous biomaterial for tissue engineering. Cryogels were produced through freeze-thawing techniques, either from agarose alone or from agarose with grafted gelatin. The aim of this study was to evaluate agarose cryogel sponges as scaffolds for Culturing both isolated pancreatic islets and insulinoma cells (INS-IE). In order to evaluate the effect of cell entrapment in artificial scaffolds, cell function reflected by insulin secretion and content was studied in cells cultivated for a 2-week period either in Culture plastic plates or in cryogel sponge disks. Our results show that tumor-derived INS-1E cells grown either on plastic or on cryogels do not differ in their proliferation, morphology, insulin release, and intracellular insulin content. However, isolated pancreatic islets cultivated on cryogels sponge show 15-fold higher basal insulin secretion at 3.0 mM glucose than islets cultivated on plastic plates and fail to respond to stimulation with 16.7 mM glucose. In addition, these islets have about 2-fold lower insulin content compared to those grown in plastic plates. It is possible that the cell dysfunction noted in these in vitro experiments is due to the effect of the limited oxygen supply to the islets cultivated in cryogel sponge. Further in vivo Studies are needed to clarify the nature of such an observation since according to previous reports, agarose and gelatin induce new vessel formation supporting enhanced oxygen supply. (c) 2005 Wiley Periodicals, Inc.},
  author       = {Bloch, K and Lozinsky, V I and Galaev, Igor and Yavriyanz, K and Vorobeychik, M and Azarov, D and Damshkaln, L G and Mattiasson, Bo and Vardi, P},
  issn         = {1552-4965},
  language     = {eng},
  number       = {4},
  pages        = {802--809},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Biomedical Materials Research. Part A},
  title        = {Functional activity of insulinoma cells (INS-1E) and pancreatic islets cultured in agarose cryogel sponges},
  url          = {http://dx.doi.org/10.1002/jbm.a.30466},
  volume       = {75A},
  year         = {2005},
}