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Profile of hydrolases and biogas production during two-stage mesophilic anaerobic digestion of solid potato waste

Parawira, Wilson LU ; Murto, Marika LU ; Read, J S and Mattiasson, Bo LU (2005) In Process Biochemistry 40(9). p.2945-2952
Abstract
Two two-stage systems, one consisting of a solid-bed reactor for hydrolysis/acidification connected to an upflow anaerobic sludge blanket methanogenic reactor, and the other consisting of a solid-bed reactor connected to a methanogenic reactor packed with wheat straw biofilm carriers, were investigated with regard to hydrolytic enzymes and methane production during mesophilic anaerobic digestion of solid potato waste. Some of the enzymes used by microorganisms to degrade the potato were found to be amylase, carboxymethyl cellulase, filter paper cellulase, xylanase, pectinase and protease. Both free and cell-bound enzyme activities were measured. The activity of the free enzyme was higher than that of the cell-bound for all the enzymes. The... (More)
Two two-stage systems, one consisting of a solid-bed reactor for hydrolysis/acidification connected to an upflow anaerobic sludge blanket methanogenic reactor, and the other consisting of a solid-bed reactor connected to a methanogenic reactor packed with wheat straw biofilm carriers, were investigated with regard to hydrolytic enzymes and methane production during mesophilic anaerobic digestion of solid potato waste. Some of the enzymes used by microorganisms to degrade the potato were found to be amylase, carboxymethyl cellulase, filter paper cellulase, xylanase, pectinase and protease. Both free and cell-bound enzyme activities were measured. The activity of the free enzyme was higher than that of the cell-bound for all the enzymes. The amylase activity was highest, followed by carboxymethyl cellulase and filter paper cellulase, while the other hydrolytic enzymes had low activities. The methane yield (0.39 m (3)/kg VSadded) and the cumulative methane production was the same in both systems. The system with packed straw bed degraded the organic matter faster than the system with the UASB methanogenic reactor. (c) 2005 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Process Biochemistry
volume
40
issue
9
pages
2945 - 2952
publisher
Elsevier
external identifiers
  • wos:000231841800002
  • scopus:22644442425
ISSN
1873-3298
DOI
10.1016/j.procbio.2005.01.010
language
English
LU publication?
yes
id
b5b0ba5e-d920-431e-a479-ba0d68843961 (old id 155049)
date added to LUP
2007-07-02 15:59:08
date last changed
2017-11-12 03:28:44
@article{b5b0ba5e-d920-431e-a479-ba0d68843961,
  abstract     = {Two two-stage systems, one consisting of a solid-bed reactor for hydrolysis/acidification connected to an upflow anaerobic sludge blanket methanogenic reactor, and the other consisting of a solid-bed reactor connected to a methanogenic reactor packed with wheat straw biofilm carriers, were investigated with regard to hydrolytic enzymes and methane production during mesophilic anaerobic digestion of solid potato waste. Some of the enzymes used by microorganisms to degrade the potato were found to be amylase, carboxymethyl cellulase, filter paper cellulase, xylanase, pectinase and protease. Both free and cell-bound enzyme activities were measured. The activity of the free enzyme was higher than that of the cell-bound for all the enzymes. The amylase activity was highest, followed by carboxymethyl cellulase and filter paper cellulase, while the other hydrolytic enzymes had low activities. The methane yield (0.39 m (3)/kg VSadded) and the cumulative methane production was the same in both systems. The system with packed straw bed degraded the organic matter faster than the system with the UASB methanogenic reactor. (c) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Parawira, Wilson and Murto, Marika and Read, J S and Mattiasson, Bo},
  issn         = {1873-3298},
  language     = {eng},
  number       = {9},
  pages        = {2945--2952},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Profile of hydrolases and biogas production during two-stage mesophilic anaerobic digestion of solid potato waste},
  url          = {http://dx.doi.org/10.1016/j.procbio.2005.01.010},
  volume       = {40},
  year         = {2005},
}