Advanced

High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format

Galaev, Igor LU ; Dainiak, Maria LU ; Plieva, Fatima LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2005) In Journal of Chromatography A 1065(2). p.169-175
Abstract
Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the... (More)
Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the cryogel monolith making the monolith columns drainage protected. The application of a certain volume of liquid on top of a cryogel monolith column results in the displacement of exactly the same volume of liquid from the column. The concept of using supermacroporous gels in 96-well plate format offers new possibilities to the biotechnologist allowing separation of particulate matter, capturing of soluble material from particle containing media, and parallel assay of large number of non-clarified samples. (C) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1065
issue
2
pages
169 - 175
publisher
Elsevier
external identifiers
  • pmid:15782962
  • wos:000227224700002
  • scopus:13644265606
ISSN
0021-9673
DOI
10.1016/j.chroma.2004.12.080
language
English
LU publication?
yes
id
42481fc4-f897-48f8-bb15-17de4a17c2ed (old id 155205)
date added to LUP
2007-07-02 10:31:42
date last changed
2017-04-09 04:20:22
@article{42481fc4-f897-48f8-bb15-17de4a17c2ed,
  abstract     = {Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the cryogel monolith making the monolith columns drainage protected. The application of a certain volume of liquid on top of a cryogel monolith column results in the displacement of exactly the same volume of liquid from the column. The concept of using supermacroporous gels in 96-well plate format offers new possibilities to the biotechnologist allowing separation of particulate matter, capturing of soluble material from particle containing media, and parallel assay of large number of non-clarified samples. (C) 2005 Elsevier B.V. All rights reserved.},
  author       = {Galaev, Igor and Dainiak, Maria and Plieva, Fatima and Hatti-Kaul, Rajni and Mattiasson, Bo},
  issn         = {0021-9673},
  language     = {eng},
  number       = {2},
  pages        = {169--175},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format},
  url          = {http://dx.doi.org/10.1016/j.chroma.2004.12.080},
  volume       = {1065},
  year         = {2005},
}