High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format
(2005) In Journal of Chromatography A 1065(2). p.169-175- Abstract
- Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the... (More)
- Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the cryogel monolith making the monolith columns drainage protected. The application of a certain volume of liquid on top of a cryogel monolith column results in the displacement of exactly the same volume of liquid from the column. The concept of using supermacroporous gels in 96-well plate format offers new possibilities to the biotechnologist allowing separation of particulate matter, capturing of soluble material from particle containing media, and parallel assay of large number of non-clarified samples. (C) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/155205
- author
- Galaev, Igor LU ; Dainiak, Maria LU ; Plieva, Fatima LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 1065
- issue
- 2
- pages
- 169 - 175
- publisher
- Elsevier
- external identifiers
-
- pmid:15782962
- wos:000227224700002
- scopus:13644265606
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2004.12.080
- language
- English
- LU publication?
- yes
- id
- 42481fc4-f897-48f8-bb15-17de4a17c2ed (old id 155205)
- date added to LUP
- 2016-04-01 16:26:48
- date last changed
- 2022-03-15 00:34:30
@article{42481fc4-f897-48f8-bb15-17de4a17c2ed,
abstract = {{Two steps in parallel processing of multiple biosamples, namely, sample clarification and capture of the target protein, were integrated and combined with the direct assay of captured protein using a newly developed microtiter (96-well) plate system based on the monoliths of hydrophilic elastic supermacroporous material, cryogel. Cryogel monoliths have pore size large enough for microbial and mammalian cells to pass through unretained. Moreover, cryogel monoliths are elastic allowing them to be slightly compressed and easily introduced into the wells. When expanded, cryogel monoliths fill the well tightly with no risk of leakage in between the monolith and the walls of the well. The capillary forces keep the liquid inside the pores of the cryogel monolith making the monolith columns drainage protected. The application of a certain volume of liquid on top of a cryogel monolith column results in the displacement of exactly the same volume of liquid from the column. The concept of using supermacroporous gels in 96-well plate format offers new possibilities to the biotechnologist allowing separation of particulate matter, capturing of soluble material from particle containing media, and parallel assay of large number of non-clarified samples. (C) 2005 Elsevier B.V. All rights reserved.}},
author = {{Galaev, Igor and Dainiak, Maria and Plieva, Fatima and Hatti-Kaul, Rajni and Mattiasson, Bo}},
issn = {{0021-9673}},
language = {{eng}},
number = {{2}},
pages = {{169--175}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{High throughput processing of particulate-containing samples using supermacroporous elastic monoliths in microtiter (multiwell) plate format}},
url = {{http://dx.doi.org/10.1016/j.chroma.2004.12.080}},
doi = {{10.1016/j.chroma.2004.12.080}},
volume = {{1065}},
year = {{2005}},
}