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Nanoparticles from Lipid-Based Liquid Crystals: Emulsifier Influence on Morphology and Cytotoxicity.

Murgia, Sergio; Falchi, Angela M; Mano, Miguel; Lampis, Sandrina; Angius, Rossella; Carnerup, Anna LU ; Schmidt, Judith; Diaz, Giacomo; Giacca, Mauro and Talmon, Yeshayahu, et al. (2010) In The Journal of Physical Chemistry Part B 114(10). p.3518-3525
Abstract
Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryo-TEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems... (More)
Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryo-TEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent. (Less)
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publication status
published
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in
The Journal of Physical Chemistry Part B
volume
114
issue
10
pages
3518 - 3525
publisher
The American Chemical Society
external identifiers
  • wos:000275328400015
  • pmid:20170140
  • scopus:77953942346
ISSN
1520-5207
DOI
10.1021/jp9098655
language
English
LU publication?
yes
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7d6f643e-9065-4655-8685-78f312852408 (old id 1552489)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20170140?dopt=Abstract
date added to LUP
2010-03-18 12:07:14
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2018-06-10 03:55:44
@article{7d6f643e-9065-4655-8685-78f312852408,
  abstract     = {Here, monoolein-based nanoparticles (NPs), obtained through fragmentation of bulk liquid crystalline phases, and stabilized by two different emulsifiers, namely, Pluronic F127 (PF127) and lauroylcholine chloride (LCh), are investigated for structural features and for short-term in vitro cytotoxicity. Depending on the emulsifiers, different morphologies of the lipid NPs (cubosomes and liposomes) are obtained, as demonstrated by cryo-TEM images. Although NPs offer many advantages in medical applications and various chemicals used for their preparation are under investigation, so far there are no standardized procedures to evaluate cell biocompatibility. Two different protocols to evaluate the impact of these lipid NPs on biological systems are presented. Results show that nanoparticles stabilized by PF127 (cubosomes) display a relevant toxicity toward different cell lines, whereas those stabilized by LCh (liposomes) affect cell viability at a much lesser extent.},
  author       = {Murgia, Sergio and Falchi, Angela M and Mano, Miguel and Lampis, Sandrina and Angius, Rossella and Carnerup, Anna and Schmidt, Judith and Diaz, Giacomo and Giacca, Mauro and Talmon, Yeshayahu and Monduzzi, Maura},
  issn         = {1520-5207},
  language     = {eng},
  number       = {10},
  pages        = {3518--3525},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Nanoparticles from Lipid-Based Liquid Crystals: Emulsifier Influence on Morphology and Cytotoxicity.},
  url          = {http://dx.doi.org/10.1021/jp9098655},
  volume       = {114},
  year         = {2010},
}