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Optimisation and robustness analysis of a hydrophobic interaction chromatography step

Jakobsson, Niklas LU ; Degerman, Marcus LU and Nilsson, Bernt LU (2005) In Journal of Chromatography A 1099(1-2). p.157-166
Abstract
Process development, optimisation and robustness analysis for chromatography separations are often entirely based on experimental work and generic knowledge. The present study proposes a method of gaining process knowledge and assisting in the robustness analysis and optimisation of a hydrophobic interaction chromatography step using a model-based approach. Factorial experimental design is common practice in industry today for robustness analysis. The method presented in this study can be used to find the critical parameter variations and serve as a basis for reducing the experimental work. In addition, the calibrated model obtained with this approach is used to find the optimal operating conditions for the chromatography column. The... (More)
Process development, optimisation and robustness analysis for chromatography separations are often entirely based on experimental work and generic knowledge. The present study proposes a method of gaining process knowledge and assisting in the robustness analysis and optimisation of a hydrophobic interaction chromatography step using a model-based approach. Factorial experimental design is common practice in industry today for robustness analysis. The method presented in this study can be used to find the critical parameter variations and serve as a basis for reducing the experimental work. In addition, the calibrated model obtained with this approach is used to find the optimal operating conditions for the chromatography column. The methodology consists of three consecutive steps. Firstly, screening experiments are performed using a factorial design. Secondly, a kinetic-dispersive model is calibrated using gradient elution and column load experiments. Finally, the model is used to find optimal operating conditions and a robustness analysis is conducted at the optimal point. The process studied in this work is the separation of polyclonal IgG from BSA using hydrophobic interaction chromatography. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chromatography A
volume
1099
issue
1-2
pages
157 - 166
publisher
Elsevier
external identifiers
  • wos:000234134400014
  • scopus:28444440181
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.09.009
language
English
LU publication?
yes
id
4d8569d0-e397-48b2-bf87-31d648553b46 (old id 156473)
date added to LUP
2007-06-25 11:53:44
date last changed
2017-09-17 07:01:12
@article{4d8569d0-e397-48b2-bf87-31d648553b46,
  abstract     = {Process development, optimisation and robustness analysis for chromatography separations are often entirely based on experimental work and generic knowledge. The present study proposes a method of gaining process knowledge and assisting in the robustness analysis and optimisation of a hydrophobic interaction chromatography step using a model-based approach. Factorial experimental design is common practice in industry today for robustness analysis. The method presented in this study can be used to find the critical parameter variations and serve as a basis for reducing the experimental work. In addition, the calibrated model obtained with this approach is used to find the optimal operating conditions for the chromatography column. The methodology consists of three consecutive steps. Firstly, screening experiments are performed using a factorial design. Secondly, a kinetic-dispersive model is calibrated using gradient elution and column load experiments. Finally, the model is used to find optimal operating conditions and a robustness analysis is conducted at the optimal point. The process studied in this work is the separation of polyclonal IgG from BSA using hydrophobic interaction chromatography.},
  author       = {Jakobsson, Niklas and Degerman, Marcus and Nilsson, Bernt},
  issn         = {0021-9673},
  language     = {eng},
  number       = {1-2},
  pages        = {157--166},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Optimisation and robustness analysis of a hydrophobic interaction chromatography step},
  url          = {http://dx.doi.org/10.1016/j.chroma.2005.09.009},
  volume       = {1099},
  year         = {2005},
}