Advanced

DNA in nanochannels-directly visualizing genomic information

Persson, Fredrik and Tegenfeldt, Jonas LU (2010) In Chemical Society Reviews 39(3). p.985-999
Abstract
The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemical Society Reviews
volume
39
issue
3
pages
985 - 999
publisher
Royal Society of Chemistry
external identifiers
  • wos:000274920300009
  • scopus:77952467722
ISSN
0306-0012
DOI
10.1039/b912918a
language
English
LU publication?
yes
id
cb65eafa-29bf-4898-a61b-ad179beaf154 (old id 1568298)
date added to LUP
2010-03-23 13:53:51
date last changed
2018-06-03 03:21:35
@article{cb65eafa-29bf-4898-a61b-ad179beaf154,
  abstract     = {The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.},
  author       = {Persson, Fredrik and Tegenfeldt, Jonas},
  issn         = {0306-0012},
  language     = {eng},
  number       = {3},
  pages        = {985--999},
  publisher    = {Royal Society of Chemistry},
  series       = {Chemical Society Reviews},
  title        = {DNA in nanochannels-directly visualizing genomic information},
  url          = {http://dx.doi.org/10.1039/b912918a},
  volume       = {39},
  year         = {2010},
}