DNA in nanochannels-directly visualizing genomic information
Persson, Fredrik and Tegenfeldt, Jonas LU
(2010)
In Chemical Society Reviews
39(3).
p.985-999
- Abstract
- The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1568298
- author
- Persson, Fredrik
and Tegenfeldt, Jonas
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chemical Society Reviews
- volume
- 39
- issue
- 3
- pages
- 985 - 999
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000274920300009
- scopus:77952467722
- pmid:20179820
- ISSN
- 0306-0012
- DOI
- 10.1039/b912918a
- language
- English
- LU publication?
- yes
- id
- cb65eafa-29bf-4898-a61b-ad179beaf154 (old id 1568298)
- date added to LUP
- 2016-04-01 10:53:38
- date last changed
- 2023-11-10 08:03:16
@article{cb65eafa-29bf-4898-a61b-ad179beaf154,
abstract = {{The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.}},
author = {{Persson, Fredrik and Tegenfeldt, Jonas}},
issn = {{0306-0012}},
language = {{eng}},
number = {{3}},
pages = {{985--999}},
publisher = {{Royal Society of Chemistry}},
series = {{Chemical Society Reviews}},
title = {{DNA in nanochannels-directly visualizing genomic information}},
url = {{http://dx.doi.org/10.1039/b912918a}},
doi = {{10.1039/b912918a}},
volume = {{39}},
year = {{2010}},
}