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Antibiograms and Randomly Amplified Polymorphic DNA-Polymerase Chain Reactions (RAPD-PCR) as Epidemiological Markers of Gonorrhea

Lawung, Ratana; Charoenwatanachokchai, Angkana; Cherdtrakulkiat, Rungrot; Thammapiwan, Sivarak; Mungniponpan, Tharinda; Bülow, Leif LU and Prachayasittikul, Virapong (2010) In Journal of Clinical Laboratory Analysis 24(1). p.31-37
Abstract
The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence... (More)
The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P<0.0001). Mutation of Ser-91 in gyrA could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD-PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5'-AGTGAATTCGCGGTGAGATGCCA-3') and D8635 (5'-GAGCGGCCAAAGGGAG-CA GAC-3'), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser-91 mutation of gyrA and RAPD-PCR methods are most useful for epidemiological screening. (C) 2010 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neissseria gonorrhoeae, gonorrhea, gyrase A, RAPD-PCR
in
Journal of Clinical Laboratory Analysis
volume
24
issue
1
pages
31 - 37
publisher
John Wiley & Sons
external identifiers
  • wos:000274403200007
  • scopus:75749097692
ISSN
1098-2825
DOI
10.1002/jcla.20355
language
English
LU publication?
yes
id
43d905df-8f32-439f-b1fb-2d918a37b79b (old id 1568916)
date added to LUP
2010-03-17 11:18:17
date last changed
2018-05-29 11:23:30
@article{43d905df-8f32-439f-b1fb-2d918a37b79b,
  abstract     = {The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P&lt;0.0001). Mutation of Ser-91 in gyrA could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD-PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5'-AGTGAATTCGCGGTGAGATGCCA-3') and D8635 (5'-GAGCGGCCAAAGGGAG-CA GAC-3'), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser-91 mutation of gyrA and RAPD-PCR methods are most useful for epidemiological screening. (C) 2010 Wiley-Liss, Inc.},
  author       = {Lawung, Ratana and Charoenwatanachokchai, Angkana and Cherdtrakulkiat, Rungrot and Thammapiwan, Sivarak and Mungniponpan, Tharinda and Bülow, Leif and Prachayasittikul, Virapong},
  issn         = {1098-2825},
  keyword      = {Neissseria gonorrhoeae,gonorrhea,gyrase A,RAPD-PCR},
  language     = {eng},
  number       = {1},
  pages        = {31--37},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Clinical Laboratory Analysis},
  title        = {Antibiograms and Randomly Amplified Polymorphic DNA-Polymerase Chain Reactions (RAPD-PCR) as Epidemiological Markers of Gonorrhea},
  url          = {http://dx.doi.org/10.1002/jcla.20355},
  volume       = {24},
  year         = {2010},
}