Antibiograms and Randomly Amplified Polymorphic DNA-Polymerase Chain Reactions (RAPD-PCR) as Epidemiological Markers of Gonorrhea
(2010) In Journal of Clinical Laboratory Analysis 24(1). p.31-37- Abstract
- The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence... (More)
- The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P<0.0001). Mutation of Ser-91 in gyrA could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD-PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5'-AGTGAATTCGCGGTGAGATGCCA-3') and D8635 (5'-GAGCGGCCAAAGGGAG-CA GAC-3'), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser-91 mutation of gyrA and RAPD-PCR methods are most useful for epidemiological screening. (C) 2010 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1568916
- author
- Lawung, Ratana ; Charoenwatanachokchai, Angkana ; Cherdtrakulkiat, Rungrot ; Thammapiwan, Sivarak ; Mungniponpan, Tharinda ; Bülow, Leif LU and Prachayasittikul, Virapong
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Neissseria gonorrhoeae, gonorrhea, gyrase A, RAPD-PCR
- in
- Journal of Clinical Laboratory Analysis
- volume
- 24
- issue
- 1
- pages
- 31 - 37
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000274403200007
- scopus:75749097692
- pmid:20087948
- ISSN
- 1098-2825
- DOI
- 10.1002/jcla.20355
- language
- English
- LU publication?
- yes
- id
- 43d905df-8f32-439f-b1fb-2d918a37b79b (old id 1568916)
- date added to LUP
- 2016-04-01 11:16:18
- date last changed
- 2025-01-14 09:41:55
@article{43d905df-8f32-439f-b1fb-2d918a37b79b, abstract = {{The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P<0.0001). Mutation of Ser-91 in gyrA could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD-PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5'-AGTGAATTCGCGGTGAGATGCCA-3') and D8635 (5'-GAGCGGCCAAAGGGAG-CA GAC-3'), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser-91 mutation of gyrA and RAPD-PCR methods are most useful for epidemiological screening. (C) 2010 Wiley-Liss, Inc.}}, author = {{Lawung, Ratana and Charoenwatanachokchai, Angkana and Cherdtrakulkiat, Rungrot and Thammapiwan, Sivarak and Mungniponpan, Tharinda and Bülow, Leif and Prachayasittikul, Virapong}}, issn = {{1098-2825}}, keywords = {{Neissseria gonorrhoeae; gonorrhea; gyrase A; RAPD-PCR}}, language = {{eng}}, number = {{1}}, pages = {{31--37}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Clinical Laboratory Analysis}}, title = {{Antibiograms and Randomly Amplified Polymorphic DNA-Polymerase Chain Reactions (RAPD-PCR) as Epidemiological Markers of Gonorrhea}}, url = {{http://dx.doi.org/10.1002/jcla.20355}}, doi = {{10.1002/jcla.20355}}, volume = {{24}}, year = {{2010}}, }