Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.
(2006) In Human Reproduction 21(2006 May 9). p.2061-2064- Abstract
- BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion... (More)
- BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI > 30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/156899
- author
- Erenpreiss, Juris LU ; Bungum, Mona LU ; Spano, M ; Elzanaty, Saad LU ; Orbidans, J and Giwercman, Aleksander LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- flow cytometry, sperm DNA integrity, SCSA, infertility, sperm chromatin
- in
- Human Reproduction
- volume
- 21
- issue
- 2006 May 9
- pages
- 2061 - 2064
- publisher
- Oxford University Press
- external identifiers
-
- wos:000239901900021
- pmid:16684843
- scopus:33747877744
- ISSN
- 0268-1161
- DOI
- 10.1093/humrep/del134
- language
- English
- LU publication?
- yes
- id
- 4ef20d23-610c-4396-b5d1-970e62dbafe7 (old id 156899)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16684843&dopt=Abstract
- date added to LUP
- 2016-04-01 11:53:33
- date last changed
- 2022-04-28 21:34:02
@article{4ef20d23-610c-4396-b5d1-970e62dbafe7, abstract = {{BACKGROUND: Sperm DNA integrity is an important factor in the prognosis of male fertility. In this study, we investigated intra-individual variation of sperm chromatin structure assay (SCSA) parameters in infertility patients undergoing assisted reproductive techniques (ARTs). METHODS: Retrospective study of 282 consecutive patients referred for ART [intrauterine insemination (IUI), IVF or ICSI] with repeated (between 2 and 5) SCSA measurements. RESULTS: Mean coefficient of variation (CV) of DNA Fragmentation Index (DFI) for repeated SCSA measurements was 29%. A high proportion [37%; 95% confidence interval (CI): 27%, 49%] of patients with DFI > 30% in the first test had DFI < 30% in the second test. Also, a considerable proportion (27%; 95% CI : 16%, 40%) of patients with 21-30% DFI values in the first test had DFI > 30% in the second test. CONCLUSIONS: Intra-individual variability in DFI is significant, therefore repeated SCSA measurements are recommended. The biological mechanisms behind these variations remain to be elucidated.}}, author = {{Erenpreiss, Juris and Bungum, Mona and Spano, M and Elzanaty, Saad and Orbidans, J and Giwercman, Aleksander}}, issn = {{0268-1161}}, keywords = {{flow cytometry; sperm DNA integrity; SCSA; infertility; sperm chromatin}}, language = {{eng}}, number = {{2006 May 9}}, pages = {{2061--2064}}, publisher = {{Oxford University Press}}, series = {{Human Reproduction}}, title = {{Intra-individual variation in sperm chromatin structure assay parameters in men from infertile couples: clinical implications.}}, url = {{http://dx.doi.org/10.1093/humrep/del134}}, doi = {{10.1093/humrep/del134}}, volume = {{21}}, year = {{2006}}, }