Advanced

Increased TLR7 expression in the adenoids among children with otitis media with effusion

Granath, Anna; Uddman, Rolf LU and Cardell, Lars-Olaf (2010) In Acta Oto-Laryngologica 130(1). p.57-61
Abstract
Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results:... (More)
Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Streptococcus pneumoniae, Haemophilus influenzae, RT-PCR, TLR4, immunohistochemistry
in
Acta Oto-Laryngologica
volume
130
issue
1
pages
57 - 61
publisher
Taylor & Francis
external identifiers
  • wos:000274416300010
  • scopus:75449108827
ISSN
1651-2251
DOI
10.3109/00016480902963061
language
English
LU publication?
yes
id
a9e4e740-6881-4e31-981d-9008fb8cc416 (old id 1569016)
date added to LUP
2010-03-17 10:19:39
date last changed
2018-05-29 12:05:24
@article{a9e4e740-6881-4e31-981d-9008fb8cc416,
  abstract     = {Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4.},
  author       = {Granath, Anna and Uddman, Rolf and Cardell, Lars-Olaf},
  issn         = {1651-2251},
  keyword      = {Streptococcus pneumoniae,Haemophilus influenzae,RT-PCR,TLR4,immunohistochemistry},
  language     = {eng},
  number       = {1},
  pages        = {57--61},
  publisher    = {Taylor & Francis},
  series       = {Acta Oto-Laryngologica},
  title        = {Increased TLR7 expression in the adenoids among children with otitis media with effusion},
  url          = {http://dx.doi.org/10.3109/00016480902963061},
  volume       = {130},
  year         = {2010},
}