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Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases

Geddes, C. C.; Peterson, J. J.; Roslander, Christian LU ; Zacchi, Guido LU ; Mullinnix, M. T.; Shanmugam, K. T. and Ingram, L. O. (2010) In Bioresource Technology 101(6). p.1851-1857
Abstract
A low level of phosphoric acid (1% w/w on dry bagasse basis, 160 degrees C and above, 10 min) was shown to effectively hydrolyze the hemicellulose in sugar cane bagasse into monomers with minimal side reactions and to serve as an effective pre-treatment for the enzymatic hydrolysis of cellulose. Up to 45% of the remaining water-insoluble solids (WIS) was digested to sugar monomers by a low concentration of Biocellulase W (0.5 filter paper unit/g WIS) supplemented with beta-glucosidase, although much higher levels of cellulase (100-fold) were required for complete hydrolysis. After neutralization and nutrient addition, phosphoric acid syrups of hemicellulose sugars were fermented by ethanologenic Escherichia coli LY160 without further... (More)
A low level of phosphoric acid (1% w/w on dry bagasse basis, 160 degrees C and above, 10 min) was shown to effectively hydrolyze the hemicellulose in sugar cane bagasse into monomers with minimal side reactions and to serve as an effective pre-treatment for the enzymatic hydrolysis of cellulose. Up to 45% of the remaining water-insoluble solids (WIS) was digested to sugar monomers by a low concentration of Biocellulase W (0.5 filter paper unit/g WIS) supplemented with beta-glucosidase, although much higher levels of cellulase (100-fold) were required for complete hydrolysis. After neutralization and nutrient addition, phosphoric acid syrups of hemicellulose sugars were fermented by ethanologenic Escherichia coli LY160 without further purification. Fermentation of these syrups was preceded by a lag that increased with increased pre-treatment temperature. Further improvements in organisms and optimization of steam treatments may allow the co-fermentation of sugars derived from hemicellulose and cellulose, eliminating need for liquid-solid separation, sugar purification, and separate fermentations. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pre-treatment, Ethanol, Lignocellulose, Xylose
in
Bioresource Technology
volume
101
issue
6
pages
1851 - 1857
publisher
Elsevier
external identifiers
  • wos:000274271400043
  • scopus:77955175376
ISSN
1873-2976
DOI
10.1016/j.biortech.2009.09.070
language
English
LU publication?
yes
id
1b32fdc0-a5b1-4613-b16c-03c3057388ca (old id 1571158)
date added to LUP
2010-03-16 14:46:33
date last changed
2018-07-15 03:41:03
@article{1b32fdc0-a5b1-4613-b16c-03c3057388ca,
  abstract     = {A low level of phosphoric acid (1% w/w on dry bagasse basis, 160 degrees C and above, 10 min) was shown to effectively hydrolyze the hemicellulose in sugar cane bagasse into monomers with minimal side reactions and to serve as an effective pre-treatment for the enzymatic hydrolysis of cellulose. Up to 45% of the remaining water-insoluble solids (WIS) was digested to sugar monomers by a low concentration of Biocellulase W (0.5 filter paper unit/g WIS) supplemented with beta-glucosidase, although much higher levels of cellulase (100-fold) were required for complete hydrolysis. After neutralization and nutrient addition, phosphoric acid syrups of hemicellulose sugars were fermented by ethanologenic Escherichia coli LY160 without further purification. Fermentation of these syrups was preceded by a lag that increased with increased pre-treatment temperature. Further improvements in organisms and optimization of steam treatments may allow the co-fermentation of sugars derived from hemicellulose and cellulose, eliminating need for liquid-solid separation, sugar purification, and separate fermentations. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Geddes, C. C. and Peterson, J. J. and Roslander, Christian and Zacchi, Guido and Mullinnix, M. T. and Shanmugam, K. T. and Ingram, L. O.},
  issn         = {1873-2976},
  keyword      = {Pre-treatment,Ethanol,Lignocellulose,Xylose},
  language     = {eng},
  number       = {6},
  pages        = {1851--1857},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Optimizing the saccharification of sugar cane bagasse using dilute phosphoric acid followed by fungal cellulases},
  url          = {http://dx.doi.org/10.1016/j.biortech.2009.09.070},
  volume       = {101},
  year         = {2010},
}