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Electrochemical and spectrophotometric studies on dyes and proteins labelled with dyes

Salomi, B S B; Mitra, C K and Gorton, Lo LU (2005) In Synthetic Metals 155(2). p.426-429
Abstract
Electrochemical and fluorometric studies on a few selected phenazine and phenoxazine dyes has been carried out in free conditions and when covalently attached to a protein. The dyes have been coupled using the conventional coupling agent carbodiimide (derivative). Neutral Red and Nile Blue A, show significant shifts in their emission peaks to shorter wavelengths on binding to bovine serum albumin (BSA). Phenazine dyes such as Safranine O and Neutral Red show a shift in their formal redox potential to more positive potential on binding to BSA along with the two electron transfers taking place in a single step unlike incase of the free dyes where the two electron transfers take place at different potentials. Nile Blue A shows a negative... (More)
Electrochemical and fluorometric studies on a few selected phenazine and phenoxazine dyes has been carried out in free conditions and when covalently attached to a protein. The dyes have been coupled using the conventional coupling agent carbodiimide (derivative). Neutral Red and Nile Blue A, show significant shifts in their emission peaks to shorter wavelengths on binding to bovine serum albumin (BSA). Phenazine dyes such as Safranine O and Neutral Red show a shift in their formal redox potential to more positive potential on binding to BSA along with the two electron transfers taking place in a single step unlike incase of the free dyes where the two electron transfers take place at different potentials. Nile Blue A shows a negative shift in the reduction peak on covalently binding to BSA. These dyes when covalently coupled to the proteins mediate intramolecular electron transfers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Synthetic Metals
volume
155
issue
2
pages
426 - 429
publisher
Elsevier
external identifiers
  • wos:000234150000046
  • scopus:28544450300
ISSN
0379-6779
DOI
10.1016/j.synthmet.2005.09.029
language
English
LU publication?
yes
id
06a785a3-4e19-41e9-8e71-eec02f14c664 (old id 157154)
date added to LUP
2007-06-27 14:27:23
date last changed
2017-04-30 14:36:09
@article{06a785a3-4e19-41e9-8e71-eec02f14c664,
  abstract     = {Electrochemical and fluorometric studies on a few selected phenazine and phenoxazine dyes has been carried out in free conditions and when covalently attached to a protein. The dyes have been coupled using the conventional coupling agent carbodiimide (derivative). Neutral Red and Nile Blue A, show significant shifts in their emission peaks to shorter wavelengths on binding to bovine serum albumin (BSA). Phenazine dyes such as Safranine O and Neutral Red show a shift in their formal redox potential to more positive potential on binding to BSA along with the two electron transfers taking place in a single step unlike incase of the free dyes where the two electron transfers take place at different potentials. Nile Blue A shows a negative shift in the reduction peak on covalently binding to BSA. These dyes when covalently coupled to the proteins mediate intramolecular electron transfers.},
  author       = {Salomi, B S B and Mitra, C K and Gorton, Lo},
  issn         = {0379-6779},
  language     = {eng},
  number       = {2},
  pages        = {426--429},
  publisher    = {Elsevier},
  series       = {Synthetic Metals},
  title        = {Electrochemical and spectrophotometric studies on dyes and proteins labelled with dyes},
  url          = {http://dx.doi.org/10.1016/j.synthmet.2005.09.029},
  volume       = {155},
  year         = {2005},
}