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Formation of supported phospholipid bilayers, via co-adsorption with beta-D-dodecyl maltoside

Vacklin, H P; Tiberg, Fredrik LU and Thomas, R K (2005) In Biochimica et Biophysica Acta - Biomembranes 1668(1). p.17-24
Abstract
We have investigated the formation of supported model membranes via the adsorption of phospholipid-surfactant mixtures at the Si water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant beta-D-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d(25)-beta-D-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39 +/- 3 (A) dot over for DOPC and 41 +/- 3 A for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 (A) dot over (2) by varying the bulk concentrations used. The amount of surfactant in the bilayer is very... (More)
We have investigated the formation of supported model membranes via the adsorption of phospholipid-surfactant mixtures at the Si water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant beta-D-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d(25)-beta-D-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39 +/- 3 (A) dot over for DOPC and 41 +/- 3 A for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 (A) dot over (2) by varying the bulk concentrations used. The amount of surfactant in the bilayer is very sensitive to the bulk volume-to-surface area ratio, but it can be fully eliminated by ensuring a sufficiently large dilution/rinsing volume of the solution. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochimica et Biophysica Acta - Biomembranes
volume
1668
issue
1
pages
17 - 24
publisher
Elsevier
external identifiers
  • scopus:12844281178
ISSN
0005-2736
DOI
10.1016/j.bbamem.2004.11.001
language
English
LU publication?
yes
id
e9cdfa85-416d-44c8-b9c9-3e6f50a9fd3b (old id 157654)
date added to LUP
2007-07-12 13:35:03
date last changed
2017-10-01 04:40:01
@article{e9cdfa85-416d-44c8-b9c9-3e6f50a9fd3b,
  abstract     = {We have investigated the formation of supported model membranes via the adsorption of phospholipid-surfactant mixtures at the Si water interface by specular neutron reflection. The adsorption of mixed micelles of the nonionic surfactant beta-D-dodecyl maltoside and DOPC or POPC was determined as a function of bulk concentration, and using d(25)-beta-D-dodecyl maltoside, the composition of DOPC and POPC bilayers was determined. Bilayer thicknesses of 39 +/- 3 (A) dot over for DOPC and 41 +/- 3 A for POPC agree well with data from bulk lamellar phases for both lipids, and the average area per lipid molecule can be varied from 62 to 115 (A) dot over (2) by varying the bulk concentrations used. The amount of surfactant in the bilayer is very sensitive to the bulk volume-to-surface area ratio, but it can be fully eliminated by ensuring a sufficiently large dilution/rinsing volume of the solution. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Vacklin, H P and Tiberg, Fredrik and Thomas, R K},
  issn         = {0005-2736},
  language     = {eng},
  number       = {1},
  pages        = {17--24},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Biomembranes},
  title        = {Formation of supported phospholipid bilayers, via co-adsorption with beta-D-dodecyl maltoside},
  url          = {http://dx.doi.org/10.1016/j.bbamem.2004.11.001},
  volume       = {1668},
  year         = {2005},
}