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MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS.

Coelho, Ana Maria Mendonça; Jukemura, José; Sampietre, Sandra N; Martins, Joilson O; Molan, Nilza A T; Patzina, Rosely A; Lindkvist, Björn LU ; Jancar, Sonia; Cunha, José Eduardo M and D'Albuquerque, Luiz A Carneiro, et al. (2010) In Shock 34. p.502-507
Abstract
BACKGROUND/AIMS:: Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are relates not only to systemic inflammatory reduction but also to reduction of the pancreatic lesion. METHODS:: AP was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: C (without AP), NT (not treated AP), NS (AP treated with NaCl 0.9%), and HS (AP treated with NaCl 7.5%). RESULTS:: Trypsinogen activation peptides (TAP) and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreas inflammation was evaluated by increased myeloperoxidase (MPO) activity,... (More)
BACKGROUND/AIMS:: Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are relates not only to systemic inflammatory reduction but also to reduction of the pancreatic lesion. METHODS:: AP was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: C (without AP), NT (not treated AP), NS (AP treated with NaCl 0.9%), and HS (AP treated with NaCl 7.5%). RESULTS:: Trypsinogen activation peptides (TAP) and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreas inflammation was evaluated by increased myeloperoxidase (MPO) activity, malonyldialdehyde (MDA) formation and histopathology for severity of pancreatic lesions. HS did not affect these parameters. Expression of cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) was markedly increased in the pancreas of the AP group and was reduced by treatment with HS. This treatment also reduced the levels of TNF-alpha and IL-6 but not of IL-10 in the pancreatic tissue. CONCLUSIONS:: These results show that HS modulates cytokines production and expression of enzymes responsible for inflammatory mediators production in the pancreas without affecting the severity of the pancreatic lesions. (Less)
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Contribution to journal
publication status
published
subject
in
Shock
volume
34
pages
502 - 507
publisher
BioMedical Press
external identifiers
  • wos:000283238700011
  • pmid:20351627
  • scopus:78149281096
ISSN
1540-0514
DOI
10.1097/SHK.0b013e3181defaa1
language
English
LU publication?
yes
id
6e76479e-dcc6-4be9-ba8f-9e7551c98121 (old id 1581430)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20351627?dopt=Abstract
date added to LUP
2010-04-07 13:51:50
date last changed
2018-05-29 11:10:14
@article{6e76479e-dcc6-4be9-ba8f-9e7551c98121,
  abstract     = {BACKGROUND/AIMS:: Administration of hypertonic saline (HS) solution to rats with acute pancreatitis (AP) decreases mortality and systemic inflammation. We hypothesized that these effects are relates not only to systemic inflammatory reduction but also to reduction of the pancreatic lesion. METHODS:: AP was induced in Wistar rats by injection of 2.5% sodium taurocholate. Animals were divided in groups: C (without AP), NT (not treated AP), NS (AP treated with NaCl 0.9%), and HS (AP treated with NaCl 7.5%). RESULTS:: Trypsinogen activation peptides (TAP) and amylase activity were increased in ascitic fluid and serum and were not affected by treatment with HS. Pancreas inflammation was evaluated by increased myeloperoxidase (MPO) activity, malonyldialdehyde (MDA) formation and histopathology for severity of pancreatic lesions. HS did not affect these parameters. Expression of cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) was markedly increased in the pancreas of the AP group and was reduced by treatment with HS. This treatment also reduced the levels of TNF-alpha and IL-6 but not of IL-10 in the pancreatic tissue. CONCLUSIONS:: These results show that HS modulates cytokines production and expression of enzymes responsible for inflammatory mediators production in the pancreas without affecting the severity of the pancreatic lesions.},
  author       = {Coelho, Ana Maria Mendonça and Jukemura, José and Sampietre, Sandra N and Martins, Joilson O and Molan, Nilza A T and Patzina, Rosely A and Lindkvist, Björn and Jancar, Sonia and Cunha, José Eduardo M and D'Albuquerque, Luiz A Carneiro and Machado, Marcel Cerqueira Cesar},
  issn         = {1540-0514},
  language     = {eng},
  pages        = {502--507},
  publisher    = {BioMedical Press},
  series       = {Shock},
  title        = {MECHANISMS OF THE BENEFICIAL EFFECT OF HYPERTONIC SALINE SOLUTION IN ACUTE PANCREATITIS.},
  url          = {http://dx.doi.org/10.1097/SHK.0b013e3181defaa1},
  volume       = {34},
  year         = {2010},
}