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Derivation of a xeno-free human ES cell line.

Ellerström, Catharina LU ; Strehl, Raimund ; Moya, Karina ; Andersson, Katarina ; Bergh, Christina ; Lundin, Kersti ; Hyllner, Johan and Semb, Henrik LU (2006) In Stem Cells 24(10). p.2170-2176
Abstract
Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (> 50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also... (More)
Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (> 50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also established xeno-free human foreskin fibroblast feeders and replaced immunosurgery, which involves the use of guinea pig complement, with a modified animal-product-free derivation procedure. Here, we report the establishment and characterization (> 20 passages) of a xeno-free pluripotent diploid normal hESC line, SA611. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
human feeders, human serum, clinical, therapies, human embryonic stem cell
in
Stem Cells
volume
24
issue
10
pages
2170 - 2176
publisher
AlphaMed Press
external identifiers
  • wos:000240965900003
  • scopus:33749538618
  • pmid:16741223
ISSN
1549-4918
DOI
10.1634/stemcells.2006-0130
language
English
LU publication?
yes
id
b326263b-b237-44c3-8b7f-beb6d20305a5 (old id 158513)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16741223&dopt=Abstract
date added to LUP
2016-04-01 16:00:32
date last changed
2021-08-04 05:27:31
@article{b326263b-b237-44c3-8b7f-beb6d20305a5,
  abstract     = {Elimination of all animal material during both the derivation and long-term culture of human embryonic stem cells (hESCs) is necessary prior to future application of hESCs in clinical cell therapy. The potential consequences of transplanting xeno-contaminated hESCs into patients, such as an increased risk of graft rejection [STEM CELLS 2006;24:221229] and the potential transfer of nonhuman pathogens, make existing hESC lines unsuitable for clinical applications. To avoid xeno-contamination during derivation and culture of hESCs, we first developed a xeno-free medium supplemented with human serum, which supports long-term (> 50 passages) culture of hESCs in an undifferentiated state. To enable derivation of new xeno-free hESCs, we also established xeno-free human foreskin fibroblast feeders and replaced immunosurgery, which involves the use of guinea pig complement, with a modified animal-product-free derivation procedure. Here, we report the establishment and characterization (> 20 passages) of a xeno-free pluripotent diploid normal hESC line, SA611.},
  author       = {Ellerström, Catharina and Strehl, Raimund and Moya, Karina and Andersson, Katarina and Bergh, Christina and Lundin, Kersti and Hyllner, Johan and Semb, Henrik},
  issn         = {1549-4918},
  language     = {eng},
  number       = {10},
  pages        = {2170--2176},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Derivation of a xeno-free human ES cell line.},
  url          = {http://dx.doi.org/10.1634/stemcells.2006-0130},
  doi          = {10.1634/stemcells.2006-0130},
  volume       = {24},
  year         = {2006},
}