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Development and evaluation of spherical molecularly imprinted polymer beads.

Kempe, Henrik LU and Kempe, Maria LU (2006) In Analytical Chemistry 78(11). p.3659-3666
Abstract
The majority of studies on molecularly imprinted polymers has until now been carried out on irregularly shaped particles prepared by grinding of polymer monoliths. The preparation procedures are time- and labor-consuming and produce particles of wide size distributions. To answer the need for fast and straightforward routes to spherical molecularly imprinted polymer beads, we have developed a method comprising the formation of droplets of pre-polymerization solution directly in mineral oil by vigorous mixing followed by transformation of the droplets into solid spherical beads by photoinduced free-radical polymerization. No detergents or stabilizers were required for the droplet formation. Factors influencing the bead synthesis have been... (More)
The majority of studies on molecularly imprinted polymers has until now been carried out on irregularly shaped particles prepared by grinding of polymer monoliths. The preparation procedures are time- and labor-consuming and produce particles of wide size distributions. To answer the need for fast and straightforward routes to spherical molecularly imprinted polymer beads, we have developed a method comprising the formation of droplets of pre-polymerization solution directly in mineral oil by vigorous mixing followed by transformation of the droplets into solid spherical beads by photoinduced free-radical polymerization. No detergents or stabilizers were required for the droplet formation. Factors influencing the bead synthesis have been investigated and are detailed here. The beads were evaluated in parallel with corresponding irregularly shaped particles prepared from polymer monoliths. Conditions for the synthesis of propranolol-imprinted poly(methacrylic acid-co-trimethylolpropane trimethacrylate) beads in the size range of 1-100 m in almost quantitative yield are described. The beads were applied as the recognition element in a 96-well plate format radioligand assay of propranolol in human serum. (Less)
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type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
78
issue
11
pages
3659 - 3666
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000237938700030
  • scopus:33744907749
  • pmid:16737221
ISSN
1520-6882
DOI
10.1021/ac060068i
language
English
LU publication?
yes
id
5d0e3a5f-bc4c-49c5-8607-04befe4b0089 (old id 158545)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16737221&dopt=Abstract
date added to LUP
2016-04-01 12:18:49
date last changed
2020-12-27 05:40:26
@article{5d0e3a5f-bc4c-49c5-8607-04befe4b0089,
  abstract     = {The majority of studies on molecularly imprinted polymers has until now been carried out on irregularly shaped particles prepared by grinding of polymer monoliths. The preparation procedures are time- and labor-consuming and produce particles of wide size distributions. To answer the need for fast and straightforward routes to spherical molecularly imprinted polymer beads, we have developed a method comprising the formation of droplets of pre-polymerization solution directly in mineral oil by vigorous mixing followed by transformation of the droplets into solid spherical beads by photoinduced free-radical polymerization. No detergents or stabilizers were required for the droplet formation. Factors influencing the bead synthesis have been investigated and are detailed here. The beads were evaluated in parallel with corresponding irregularly shaped particles prepared from polymer monoliths. Conditions for the synthesis of propranolol-imprinted poly(methacrylic acid-co-trimethylolpropane trimethacrylate) beads in the size range of 1-100 m in almost quantitative yield are described. The beads were applied as the recognition element in a 96-well plate format radioligand assay of propranolol in human serum.},
  author       = {Kempe, Henrik and Kempe, Maria},
  issn         = {1520-6882},
  language     = {eng},
  number       = {11},
  pages        = {3659--3666},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Analytical Chemistry},
  title        = {Development and evaluation of spherical molecularly imprinted polymer beads.},
  url          = {http://dx.doi.org/10.1021/ac060068i},
  doi          = {10.1021/ac060068i},
  volume       = {78},
  year         = {2006},
}