Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection
Evilevitch, Alex LU
; Gober, J
; Phillips, M
; Knobler, C
and Gelbart, W
(2005)
In Biophysical Journal
88(1).
p.751-756
- Abstract
- The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA... (More)
- The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/158582
- author
- Evilevitch, Alex
LU
; Gober, J
; Phillips, M
; Knobler, C
and Gelbart, W
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biophysical Journal
- volume
- 88
- issue
- 1
- pages
- 751 - 756
- publisher
- Cell Press
- external identifiers
-
- wos:000226090900068
- pmid:15489301
- scopus:11244340515
- pmid:15489301
- ISSN
- 1542-0086
- DOI
- 10.1529/biophysj.104.045088
- language
- English
- LU publication?
- no
- id
- ddf15b52-eb7a-489e-b445-aa7f15da4d9d (old id 158582)
- date added to LUP
- 2016-04-01 11:40:27
- date last changed
- 2022-01-26 08:33:45
@article{ddf15b52-eb7a-489e-b445-aa7f15da4d9d,
abstract = {{The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.}},
author = {{Evilevitch, Alex and Gober, J and Phillips, M and Knobler, C and Gelbart, W}},
issn = {{1542-0086}},
language = {{eng}},
number = {{1}},
pages = {{751--756}},
publisher = {{Cell Press}},
series = {{Biophysical Journal}},
title = {{Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection}},
url = {{http://dx.doi.org/10.1529/biophysj.104.045088}},
doi = {{10.1529/biophysj.104.045088}},
volume = {{88}},
year = {{2005}},
}