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Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection

Evilevitch, Alex LU ; Gober, J; Phillips, M; Knobler, C and Gelbart, W (2005) In Biophysical Journal 88(1). p.751-756
Abstract
The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA... (More)
The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
88
issue
1
pages
751 - 756
publisher
Cell Press
external identifiers
  • wos:000226090900068
  • pmid:15489301
  • scopus:11244340515
ISSN
1542-0086
DOI
10.1529/biophysj.104.045088
language
English
LU publication?
no
id
ddf15b52-eb7a-489e-b445-aa7f15da4d9d (old id 158582)
date added to LUP
2007-07-05 08:43:20
date last changed
2017-08-20 03:29:49
@article{ddf15b52-eb7a-489e-b445-aa7f15da4d9d,
  abstract     = {The effect of external osmotic pressure on the extent of DNA ejection from bacteriophage- was recently investigated (Evilevitch et al., 2003). The total length of DNA ejected was measured via the 260-nm absorption by free nucleotides, after opening of the capsids in the presence of varying amounts of polyethylene glycol 8000 and DNase I. As a function of osmolyte concentration, this absorption was shown to decrease progressively, ultimately vanishing completely for a sufficiently high external osmotic pressure. In this work we report the results of both sedimentation and gel analysis of the length of DNA remaining inside the capsids, as a function of osmolyte concentration. It is confirmed in this way that the progressive inhibition of DNA ejection corresponds to partial ejection from all of the capsids.},
  author       = {Evilevitch, Alex and Gober, J and Phillips, M and Knobler, C and Gelbart, W},
  issn         = {1542-0086},
  language     = {eng},
  number       = {1},
  pages        = {751--756},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Measurements of DNA Lengths Remaining in a Viral Capsid after Osmotically Suppressed Partial Ejection},
  url          = {http://dx.doi.org/10.1529/biophysj.104.045088},
  volume       = {88},
  year         = {2005},
}