Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Increased Kit/SCF receptor induced mitogenicity but abolished cell motility after inhibition of protein kinase C

Blume-Jensen, Peter ; Siegbahn, Agneta ; Stabel, Silvia ; Heldin, Carl-Henrik and Rönnstrand, Lars LU orcid (1993) In EMBO Journal 12(11). p.4199-4209
Abstract
The product of the c-kit proto-oncogene, denoted Kit/SCF-R, encodes a tyrosine kinase receptor for stem cell factor (SCF). Kit/SCF-R induces proliferation, differentiation or migration of cells within the hematopoietic, gametogenic and melanogenic lineages at different developmental stages. We report here that protein kinase C (PKC) mediates phosphorylation of Kit/SCF-R on serine residues in response to SCF or PMA in intact cells. The phosphorylation inhibits SCF-induced tyrosine autophosphorylation of Kit/SCF-R. In vitro studies showed that PKC phosphorylated the Kit/SCF-R directly on serine residues and inhibited autophosphorylation of Kit/SCF-R, as well as its kinase activity towards an exogenous substrate. The PKC-induced... (More)
The product of the c-kit proto-oncogene, denoted Kit/SCF-R, encodes a tyrosine kinase receptor for stem cell factor (SCF). Kit/SCF-R induces proliferation, differentiation or migration of cells within the hematopoietic, gametogenic and melanogenic lineages at different developmental stages. We report here that protein kinase C (PKC) mediates phosphorylation of Kit/SCF-R on serine residues in response to SCF or PMA in intact cells. The phosphorylation inhibits SCF-induced tyrosine autophosphorylation of Kit/SCF-R. In vitro studies showed that PKC phosphorylated the Kit/SCF-R directly on serine residues and inhibited autophosphorylation of Kit/SCF-R, as well as its kinase activity towards an exogenous substrate. The PKC-induced phosphorylation did not affect Kit/SCF-R ligand binding affinity. Inhibition of PKC led to increased SCF-induced tyrosine autophosphorylation, as well as increased SCF-induced mitogenicity. In contrast, PKC was necessary for SCF-induced motility responses, including actin reorganization and chemotaxis. Our data suggest that PKC is involved in a negative feedback loop which regulates the Kit/SCF-R and that the activity of PKC determines whether the effect of SCF will be preferentially mitogenic or motogenic. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Vascular/drug effects/enzymology/*growth & developmentHematopoietic Cell Growth Factors/pharmacologyHumans*NaphthalenesPhosphorylationPolycyclic Compounds/pharmacologyProtein Kinase C/antagonists & inhibitors/*metabolismProto-Oncogene Proteins/*metabolismProto-Oncogene Proteins c-kitReceptor Protein-Tyrosine Kinases/*metabolismReceptors, CulturedChemotaxisEndothelium, AnimalsAorta/cytologyCell Division/drug effects*Cell Movement/drug effectsCells, Colony-Stimulating Factor/*metabolismSerine/metabolismStem Cell FactorSwine
in
EMBO Journal
volume
12
issue
11
pages
4199 - 4209
publisher
Oxford University Press
external identifiers
  • scopus:0027508336
ISSN
1460-2075
language
English
LU publication?
no
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Clinical Chemistry (013016010)
id
1585a467-b048-4ee9-acc2-ae5d0822cc87 (old id 1784137)
alternative location
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC413714/
date added to LUP
2016-04-04 09:23:29
date last changed
2021-03-14 05:44:22
@article{1585a467-b048-4ee9-acc2-ae5d0822cc87,
  abstract     = {{The product of the c-kit proto-oncogene, denoted Kit/SCF-R, encodes a tyrosine kinase receptor for stem cell factor (SCF). Kit/SCF-R induces proliferation, differentiation or migration of cells within the hematopoietic, gametogenic and melanogenic lineages at different developmental stages. We report here that protein kinase C (PKC) mediates phosphorylation of Kit/SCF-R on serine residues in response to SCF or PMA in intact cells. The phosphorylation inhibits SCF-induced tyrosine autophosphorylation of Kit/SCF-R. In vitro studies showed that PKC phosphorylated the Kit/SCF-R directly on serine residues and inhibited autophosphorylation of Kit/SCF-R, as well as its kinase activity towards an exogenous substrate. The PKC-induced phosphorylation did not affect Kit/SCF-R ligand binding affinity. Inhibition of PKC led to increased SCF-induced tyrosine autophosphorylation, as well as increased SCF-induced mitogenicity. In contrast, PKC was necessary for SCF-induced motility responses, including actin reorganization and chemotaxis. Our data suggest that PKC is involved in a negative feedback loop which regulates the Kit/SCF-R and that the activity of PKC determines whether the effect of SCF will be preferentially mitogenic or motogenic.}},
  author       = {{Blume-Jensen, Peter and Siegbahn, Agneta and Stabel, Silvia and Heldin, Carl-Henrik and Rönnstrand, Lars}},
  issn         = {{1460-2075}},
  keywords     = {{Vascular/drug effects/enzymology/*growth & developmentHematopoietic Cell Growth Factors/pharmacologyHumans*NaphthalenesPhosphorylationPolycyclic Compounds/pharmacologyProtein Kinase C/antagonists & inhibitors/*metabolismProto-Oncogene Proteins/*metabolismProto-Oncogene Proteins c-kitReceptor Protein-Tyrosine Kinases/*metabolismReceptors; CulturedChemotaxisEndothelium; AnimalsAorta/cytologyCell Division/drug effects*Cell Movement/drug effectsCells; Colony-Stimulating Factor/*metabolismSerine/metabolismStem Cell FactorSwine}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{4199--4209}},
  publisher    = {{Oxford University Press}},
  series       = {{EMBO Journal}},
  title        = {{Increased Kit/SCF receptor induced mitogenicity but abolished cell motility after inhibition of protein kinase C}},
  url          = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC413714/}},
  volume       = {{12}},
  year         = {{1993}},
}