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Identification of Colletotrichum species associated with anthracnose disease of coffee in Vietnam

Phuong, Thi Hang Nguyen; Pettersson, Olga Vinnere; Olsson, Peter LU and Liljeroth, Erland (2010) In European Journal of Plant Pathology 127(1). p.73-87
Abstract
Twenty-three isolates of Colletotrichum gloeosporioides, five isolates of C. acutatum, two isolates of C. capsici and six isolates of C. boninense associated with anthracnose disease on coffee (Coffea spp.) in Vietnam were identified based on morphology and DNA analysis. Phylogenetic analysis of DNA sequences from the internal transcribed spacer region of nuclear rDNA and a portion of mitochondrial small subunit rRNA were concordant and allowed good separation of the taxa. We found several Colletotrichum isolates of unknown species and their taxonomic position remains unresolved. The majority of Vietnamese isolates belonged to C. gloeosporioides and they grouped together with the coffee berry disease (CBD) fungus, C. kahawae. However, C.... (More)
Twenty-three isolates of Colletotrichum gloeosporioides, five isolates of C. acutatum, two isolates of C. capsici and six isolates of C. boninense associated with anthracnose disease on coffee (Coffea spp.) in Vietnam were identified based on morphology and DNA analysis. Phylogenetic analysis of DNA sequences from the internal transcribed spacer region of nuclear rDNA and a portion of mitochondrial small subunit rRNA were concordant and allowed good separation of the taxa. We found several Colletotrichum isolates of unknown species and their taxonomic position remains unresolved. The majority of Vietnamese isolates belonged to C. gloeosporioides and they grouped together with the coffee berry disease (CBD) fungus, C. kahawae. However, C. kahawae could be distinguished from the Vietnamese C. gloeosporioides isolates based on ammonium tartrate utilization, growth rate and pathogenicity. C. gloeosporioides isolates were more pathogenic on detached green berries than isolates of the other species, i.e. C. acutatum, C capsici and C. boninense. Some of the C. gloeosporioides isolates produced slightly sunken lesions on green berries resembling CBD symptoms but it did not destroy the bean. We did not find any evidence of the presence of C. kahawae in Vietnam. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
test, Pathogenicity, Coffee berry anthracnose, Colletotrichum gloeosporioides, Phylogenetic analysis, Tartrate metabolism
in
European Journal of Plant Pathology
volume
127
issue
1
pages
73 - 87
publisher
Kluwer
external identifiers
  • wos:000276070500009
  • scopus:77950022912
ISSN
0929-1873
DOI
10.1007/s10658-009-9573-5
language
English
LU publication?
yes
id
02d8a3a9-cbda-49be-8486-2f5452ea42c8 (old id 1587086)
date added to LUP
2010-04-27 10:30:17
date last changed
2018-05-29 11:51:10
@article{02d8a3a9-cbda-49be-8486-2f5452ea42c8,
  abstract     = {Twenty-three isolates of Colletotrichum gloeosporioides, five isolates of C. acutatum, two isolates of C. capsici and six isolates of C. boninense associated with anthracnose disease on coffee (Coffea spp.) in Vietnam were identified based on morphology and DNA analysis. Phylogenetic analysis of DNA sequences from the internal transcribed spacer region of nuclear rDNA and a portion of mitochondrial small subunit rRNA were concordant and allowed good separation of the taxa. We found several Colletotrichum isolates of unknown species and their taxonomic position remains unresolved. The majority of Vietnamese isolates belonged to C. gloeosporioides and they grouped together with the coffee berry disease (CBD) fungus, C. kahawae. However, C. kahawae could be distinguished from the Vietnamese C. gloeosporioides isolates based on ammonium tartrate utilization, growth rate and pathogenicity. C. gloeosporioides isolates were more pathogenic on detached green berries than isolates of the other species, i.e. C. acutatum, C capsici and C. boninense. Some of the C. gloeosporioides isolates produced slightly sunken lesions on green berries resembling CBD symptoms but it did not destroy the bean. We did not find any evidence of the presence of C. kahawae in Vietnam.},
  author       = {Phuong, Thi Hang Nguyen and Pettersson, Olga Vinnere and Olsson, Peter and Liljeroth, Erland},
  issn         = {0929-1873},
  keyword      = {test,Pathogenicity,Coffee berry anthracnose,Colletotrichum gloeosporioides,Phylogenetic analysis,Tartrate metabolism},
  language     = {eng},
  number       = {1},
  pages        = {73--87},
  publisher    = {Kluwer},
  series       = {European Journal of Plant Pathology},
  title        = {Identification of Colletotrichum species associated with anthracnose disease of coffee in Vietnam},
  url          = {http://dx.doi.org/10.1007/s10658-009-9573-5},
  volume       = {127},
  year         = {2010},
}