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Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry

Carlsson, J; Mecklenburg, M; Lundström, I; Danielsson, Bengt LU and Winquist, F (2005) In Analytica Chimica Acta 530(2). p.167-171
Abstract
Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
530
issue
2
pages
167 - 171
publisher
Elsevier
external identifiers
  • wos:000227192800001
  • scopus:13444261098
ISSN
1873-4324
DOI
10.1016/j.aca.2004.09.022
language
English
LU publication?
yes
id
f488d5ec-9c05-4382-b6c9-101cc4fb3838 (old id 158724)
date added to LUP
2007-07-16 08:45:57
date last changed
2017-01-01 06:40:25
@article{f488d5ec-9c05-4382-b6c9-101cc4fb3838,
  abstract     = {Serum proteins of different species and of different human blood groups exhibit various protein glycosylation patterns. Sera from human, pig, sheep and guinea pig have been applied to a panel of eight different lectins immobilized on a gold wafer. The biorecognition has been evaluated with scanning ellipsometry and the two-dimensional matrices obtained have been treated with image analysis and MVDA for evaluation. The results showed a clear difference in protein binding pattern between the different species and thereby separation of the different sera could be made. Dendograms indicate that human and pig sera are the most related of the four different sera investigated.},
  author       = {Carlsson, J and Mecklenburg, M and Lundström, I and Danielsson, Bengt and Winquist, F},
  issn         = {1873-4324},
  language     = {eng},
  number       = {2},
  pages        = {167--171},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Investigation of sera from various species by using lectin affinity arrays and scanning ellipsometry},
  url          = {http://dx.doi.org/10.1016/j.aca.2004.09.022},
  volume       = {530},
  year         = {2005},
}