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Detection of C-Reactive Protein Utilizing Magnetic Permeability Detection Based Immunoassays

Kriz, Kirstin LU ; Ibraimi, Filiz LU ; Lu, M; Hansson, L-O and Kriz, Dario (2005) In Analytical Chemistry 77(18). p.5920-5924
Abstract
A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass... (More)
A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass capillary and mixing its contents by hand for 30 s. After a 11-min sedimentation step, the vial was placed into the coil of the magnetic permeability detector, which measured the enrichment of superparamagnetic nanoparticles in the solid-phase sediment. Magnetic permeability detection and quantification is based on the principle that when paramagnetic materials are placed inside a coil, the inductance of the coil is influenced. Screening of CRP on whole blood patient samples showed good correlation with central hospital measurements for hsCRP (y = 1.018x - 0.021, R2 = 0.980, n = 103) and normal range CRP (y = 1.02x + 2.53, R2= 0.991, n = 33) analyses. The mean differences of the two methods according to the Bland and Altman plots were -0.03 ± 1.12 mg/L for hsCRP analysis and -3.4 ± 8.64 mg/L for normal range CRP analysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
77
issue
18
pages
5920 - 5924
publisher
The American Chemical Society
external identifiers
  • wos:000231919400017
  • scopus:24944545567
ISSN
1520-6882
DOI
10.1021/ac0508649
language
English
LU publication?
yes
id
5815670e-154c-4e90-a177-8bc4e39200b9 (old id 158741)
date added to LUP
2007-07-16 09:50:02
date last changed
2017-11-19 03:30:15
@article{5815670e-154c-4e90-a177-8bc4e39200b9,
  abstract     = {A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass capillary and mixing its contents by hand for 30 s. After a 11-min sedimentation step, the vial was placed into the coil of the magnetic permeability detector, which measured the enrichment of superparamagnetic nanoparticles in the solid-phase sediment. Magnetic permeability detection and quantification is based on the principle that when paramagnetic materials are placed inside a coil, the inductance of the coil is influenced. Screening of CRP on whole blood patient samples showed good correlation with central hospital measurements for hsCRP (y = 1.018x - 0.021, R2 = 0.980, n = 103) and normal range CRP (y = 1.02x + 2.53, R2= 0.991, n = 33) analyses. The mean differences of the two methods according to the Bland and Altman plots were -0.03 ± 1.12 mg/L for hsCRP analysis and -3.4 ± 8.64 mg/L for normal range CRP analysis.},
  author       = {Kriz, Kirstin and Ibraimi, Filiz and Lu, M and Hansson, L-O and Kriz, Dario},
  issn         = {1520-6882},
  language     = {eng},
  number       = {18},
  pages        = {5920--5924},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Detection of C-Reactive Protein Utilizing Magnetic Permeability Detection Based Immunoassays},
  url          = {http://dx.doi.org/10.1021/ac0508649},
  volume       = {77},
  year         = {2005},
}