Detection of C-Reactive Protein Utilizing Magnetic Permeability Detection Based Immunoassays
(2005) In Analytical Chemistry 77(18). p.5920-5924- Abstract
- A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass... (More)
- A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass capillary and mixing its contents by hand for 30 s. After a 11-min sedimentation step, the vial was placed into the coil of the magnetic permeability detector, which measured the enrichment of superparamagnetic nanoparticles in the solid-phase sediment. Magnetic permeability detection and quantification is based on the principle that when paramagnetic materials are placed inside a coil, the inductance of the coil is influenced. Screening of CRP on whole blood patient samples showed good correlation with central hospital measurements for hsCRP (y = 1.018x - 0.021, R2 = 0.980, n = 103) and normal range CRP (y = 1.02x + 2.53, R2= 0.991, n = 33) analyses. The mean differences of the two methods according to the Bland and Altman plots were -0.03 ± 1.12 mg/L for hsCRP analysis and -3.4 ± 8.64 mg/L for normal range CRP analysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/158741
- author
- Kriz, Kirstin LU ; Ibraimi, Filiz LU ; Lu, M ; Hansson, L-O and Kriz, Dario
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 77
- issue
- 18
- pages
- 5920 - 5924
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000231919400017
- scopus:24944545567
- pmid:16159122
- ISSN
- 1520-6882
- DOI
- 10.1021/ac0508649
- language
- English
- LU publication?
- yes
- id
- 5815670e-154c-4e90-a177-8bc4e39200b9 (old id 158741)
- date added to LUP
- 2016-04-01 11:55:47
- date last changed
- 2022-01-26 20:21:39
@article{5815670e-154c-4e90-a177-8bc4e39200b9,
abstract = {{A new sensing technology platform integrating magnetic permeability detection and a two-site heterogeneous immunoassay in a one-step analysis is described. As a platform model, measurements of C-reactive protein (CRP), a cardiac and inflammation marker, were performed in a rapid (11.5 min) high-sensitivity (hs) procedure with a low detection limit (0.2 mg/L) and accuracy (CV = 11%). The two-site heterogeneous immunoassay was performed in 1.2-mL disposable reagents vials containing solid phase (polyclonal anti-CRP conjugated silica microparticles), labeling agent (monoclonal anti-CRP conjugated superparamagnetic nanoparticles), and reaction buffer. Whole blood (20 L) was assayed by introducing the sample into a reagent vial using a glass capillary and mixing its contents by hand for 30 s. After a 11-min sedimentation step, the vial was placed into the coil of the magnetic permeability detector, which measured the enrichment of superparamagnetic nanoparticles in the solid-phase sediment. Magnetic permeability detection and quantification is based on the principle that when paramagnetic materials are placed inside a coil, the inductance of the coil is influenced. Screening of CRP on whole blood patient samples showed good correlation with central hospital measurements for hsCRP (y = 1.018x - 0.021, R2 = 0.980, n = 103) and normal range CRP (y = 1.02x + 2.53, R2= 0.991, n = 33) analyses. The mean differences of the two methods according to the Bland and Altman plots were -0.03 ± 1.12 mg/L for hsCRP analysis and -3.4 ± 8.64 mg/L for normal range CRP analysis.}},
author = {{Kriz, Kirstin and Ibraimi, Filiz and Lu, M and Hansson, L-O and Kriz, Dario}},
issn = {{1520-6882}},
language = {{eng}},
number = {{18}},
pages = {{5920--5924}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Detection of C-Reactive Protein Utilizing Magnetic Permeability Detection Based Immunoassays}},
url = {{http://dx.doi.org/10.1021/ac0508649}},
doi = {{10.1021/ac0508649}},
volume = {{77}},
year = {{2005}},
}