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Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens

Garcia-Caballero, Tomas; Grabau, Dorthe LU ; Green, Andrew R.; Gregory, John; Schad, Arno; Kohlwes, Elke; Ellis, Ian O.; Watts, Sarah and Mollerup, Jens (2010) In Histopathology 56(4). p.472-480
Abstract
Aims: Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. Methods and results: We found 100% agreement between HER2 status determined by FISH and dual-colour CISH. Furthermore, we observed that the... (More)
Aims: Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. Methods and results: We found 100% agreement between HER2 status determined by FISH and dual-colour CISH. Furthermore, we observed that the time used to score slides was significantly reduced by 28% in dual-colour CISH compared with the FISH protocol. Concordance between HER2 protein status and dual-colour CISH or FISH was equally good with an overall agreement of 96.8%. Correlation between the HER2/centromere 17 gene ratios obtained with dual-colour CISH and FISH was highly significant with an overall correlation coefficient (rho) of 0.96. Conclusions: We conclude that dual-colour CISH and bright field microscopy are excellent alternatives to FISH when analysing the HER2 status of primary breast cancer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hybridization, in situ, HER2 amplification, HER2, FISH, CISH, breast cancer, CEN-17
in
Histopathology
volume
56
issue
4
pages
472 - 480
publisher
Wiley-Blackwell
external identifiers
  • wos:000275676000007
  • scopus:77949591945
ISSN
0309-0167
DOI
10.1111/j.1365-2559.2010.03503.x
language
English
LU publication?
yes
id
c43ec29b-14c5-4ec1-978c-69a8cd2092df (old id 1588009)
date added to LUP
2010-04-22 11:33:46
date last changed
2018-05-29 10:22:56
@article{c43ec29b-14c5-4ec1-978c-69a8cd2092df,
  abstract     = {Aims: Fluorescence in situ hybridization (FISH) can be used to reveal several genomic imbalances relevant to proper cancer diagnosis and to the correct treatment regime. However, FISH requires expensive and advanced fluorescence microscopes in addition to expertise in fluorescence microscopy. To determine whether a newly developed dual-colour chromogenic in situ hybridization (CISH) method is a suitable alternative to FISH, we analysed the human epidermal growth factor receptor 2 gene (HER2) amplification level of 168 breast cancer specimens using dual-colour CISH and FISH and compared the results. Methods and results: We found 100% agreement between HER2 status determined by FISH and dual-colour CISH. Furthermore, we observed that the time used to score slides was significantly reduced by 28% in dual-colour CISH compared with the FISH protocol. Concordance between HER2 protein status and dual-colour CISH or FISH was equally good with an overall agreement of 96.8%. Correlation between the HER2/centromere 17 gene ratios obtained with dual-colour CISH and FISH was highly significant with an overall correlation coefficient (rho) of 0.96. Conclusions: We conclude that dual-colour CISH and bright field microscopy are excellent alternatives to FISH when analysing the HER2 status of primary breast cancer.},
  author       = {Garcia-Caballero, Tomas and Grabau, Dorthe and Green, Andrew R. and Gregory, John and Schad, Arno and Kohlwes, Elke and Ellis, Ian O. and Watts, Sarah and Mollerup, Jens},
  issn         = {0309-0167},
  keyword      = {hybridization,in situ,HER2 amplification,HER2,FISH,CISH,breast cancer,CEN-17},
  language     = {eng},
  number       = {4},
  pages        = {472--480},
  publisher    = {Wiley-Blackwell},
  series       = {Histopathology},
  title        = {Determination of HER2 amplification in primary breast cancer using dual-colour chromogenic in situ hybridization is comparable to fluorescence in situ hybridization: a European multicentre study involving 168 specimens},
  url          = {http://dx.doi.org/10.1111/j.1365-2559.2010.03503.x},
  volume       = {56},
  year         = {2010},
}