Advanced

Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination

Tufvesson, Ellen LU ; van Weele, Linda Jacqueline; Ekedahl, Henrik and Bjermer, Leif LU (2010) In Clinical Respiratory Journal 4(2). p.83-88
Abstract
Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and... (More)
Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
exhaled breath, cysteinyl-leukotrienes, alpha-amylase, asthma, saliva, sputum, condensate
in
Clinical Respiratory Journal
volume
4
issue
2
pages
83 - 88
publisher
Wiley-Blackwell
external identifiers
  • wos:000275766600004
  • pmid:20565481
  • scopus:77949881057
ISSN
1752-6981
DOI
10.1111/j.1752-699X.2009.00151.x
language
English
LU publication?
yes
id
416d493d-c08e-4c43-923d-e32205681944 (old id 1588105)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20565481?dopt=Abstract
date added to LUP
2010-04-22 10:24:12
date last changed
2018-05-29 11:55:10
@article{416d493d-c08e-4c43-923d-e32205681944,
  abstract     = {Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88.},
  author       = {Tufvesson, Ellen and van Weele, Linda Jacqueline and Ekedahl, Henrik and Bjermer, Leif},
  issn         = {1752-6981},
  keyword      = {exhaled breath,cysteinyl-leukotrienes,alpha-amylase,asthma,saliva,sputum,condensate},
  language     = {eng},
  number       = {2},
  pages        = {83--88},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical Respiratory Journal},
  title        = {Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination},
  url          = {http://dx.doi.org/10.1111/j.1752-699X.2009.00151.x},
  volume       = {4},
  year         = {2010},
}