Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination
(2010) In Clinical Respiratory Journal 4(2). p.83-88- Abstract
- Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and... (More)
- Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1588105
- author
- Tufvesson, Ellen LU ; van Weele, Linda Jacqueline ; Ekedahl, Henrik LU and Bjermer, Leif LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- exhaled breath, cysteinyl-leukotrienes, alpha-amylase, asthma, saliva, sputum, condensate
- in
- Clinical Respiratory Journal
- volume
- 4
- issue
- 2
- pages
- 83 - 88
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000275766600004
- pmid:20565481
- scopus:77949881057
- pmid:20565481
- ISSN
- 1752-6981
- DOI
- 10.1111/j.1752-699X.2009.00151.x
- language
- English
- LU publication?
- yes
- id
- 416d493d-c08e-4c43-923d-e32205681944 (old id 1588105)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20565481?dopt=Abstract
- date added to LUP
- 2016-04-01 10:38:22
- date last changed
- 2022-01-26 01:07:41
@article{416d493d-c08e-4c43-923d-e32205681944, abstract = {{Background and Aims: Saliva contamination has been suggested to be a major contributor to levels of cysteinyl leukotrienes in exhaled breath condensate (EBC). The aim of this study was to compare the levels of cysteinyl-leukotrienes (CysLT) and alpha-amylase activity in EBC to induced sputum and saliva collected from the same subjects (asthmatics and control). We thereby aimed to find out whether saliva contamination could be a plausible explanation to the levels found in EBC or not. Methods: EBC, saliva and induced sputum were collected from 11 asthmatic and 19 healthy adults. These samples were analyzed for CysLT concentration and alpha-amylase activity. Results: No significant correlation was found between CysLT concentration and alpha-amylase activity in EBC, saliva or sputum. In addition, we show that the saliva contamination (measured as alpha-amylase activity) was negligible, as the relative amount of saliva CysLT was only 0.6% of that found in EBC. The amount of CysLT correlated between all three compartments (EBC, saliva and sputum), but no similar correlation was seen for the alpha-amylase activity in EBC compared to saliva and sputum. The levels of CysLT were higher in asthmatic patients compared to healthy controls in EBC, saliva and sputum. Conclusion: We conclude that the amount of CysLT in EBC cannot be explained by saliva contamination. Please cite this paper as: Tufvesson E, van Weele LJ, Ekedahl H and Bjermer L. Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination. The Clinical Respiratory Journal 2010; 4: 83-88.}}, author = {{Tufvesson, Ellen and van Weele, Linda Jacqueline and Ekedahl, Henrik and Bjermer, Leif}}, issn = {{1752-6981}}, keywords = {{exhaled breath; cysteinyl-leukotrienes; alpha-amylase; asthma; saliva; sputum; condensate}}, language = {{eng}}, number = {{2}}, pages = {{83--88}}, publisher = {{Wiley-Blackwell}}, series = {{Clinical Respiratory Journal}}, title = {{Levels of cysteinyl-leukotrienes in exhaled breath condensate are not due to saliva contamination}}, url = {{http://dx.doi.org/10.1111/j.1752-699X.2009.00151.x}}, doi = {{10.1111/j.1752-699X.2009.00151.x}}, volume = {{4}}, year = {{2010}}, }