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Comparative Molecular Evolution of Trichoderma Chitinases in Response to Mycoparasitic Interactions

Ihrmark, Katarina; Asmail, Nashwan; Ubhayasekera, Wimal LU ; Melin, Petter; Stenlid, Jan and Karlsson, Magnus (2010) In Evolutionary Bioinformatics 6. p.1-25
Abstract
Certain species of the fungal genus Trichoderma are potent mycoparasites and are used for biological control of fungal diseases on agricultural crops. In Trichoderma, whole-genome sequencing reveal between 20 and 36 different genes encoding chitinases, hydrolytic enzymes that are involved in the mycoparasitic attack. Sequences of Trichoderma chitinase genes chi18-5, chi18-13, chi18-15 and chi18-17, which all exhibit specific expression during mycoparasitism-related conditions, were determined from up to 13 different taxa and studied with regard to their evolutionary patterns. Two of them, chi18-13 and chi18-17, are members of the B1/B2 chitinase subgroup that have expanded significantly in paralog number in mycoparasitic Hypocrea... (More)
Certain species of the fungal genus Trichoderma are potent mycoparasites and are used for biological control of fungal diseases on agricultural crops. In Trichoderma, whole-genome sequencing reveal between 20 and 36 different genes encoding chitinases, hydrolytic enzymes that are involved in the mycoparasitic attack. Sequences of Trichoderma chitinase genes chi18-5, chi18-13, chi18-15 and chi18-17, which all exhibit specific expression during mycoparasitism-related conditions, were determined from up to 13 different taxa and studied with regard to their evolutionary patterns. Two of them, chi18-13 and chi18-17, are members of the B1/B2 chitinase subgroup that have expanded significantly in paralog number in mycoparasitic Hypocrea atroviridis and H. virens. Chi18-13 contains two codons that evolve under positive selection and seven groups of co-evolving sites. Chi18-15 displays a unique codon-usage and contains five codons that evolve under positive selection and three groups of co-evolving sites. Regions of high amino acid variability are preferentially localized to substrate-or product side of the catalytic clefts. Differences in amino acid diversity/conservation patterns between different Trichoderma clades are observed. These observations show that Trichoderma chitinases chi18-13 and chi18-15 evolve in a manner consistent with rapid co-evolutionary interactions and identifies putative target regions involved in determining substrate-specificity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chitinase, mycoparasitism, protein evolution, Trichoderma
in
Evolutionary Bioinformatics
volume
6
pages
1 - 25
publisher
Libertas Academica
external identifiers
  • wos:000275600100001
  • scopus:77951266103
ISSN
1176-9343
language
English
LU publication?
yes
id
d56fed6c-21fd-448f-a428-3cb4366dceec (old id 1588428)
alternative location
http://www.la-press.com/comparative-molecular-evolution-of-trichoderma-chitinases-in-response--a1919
date added to LUP
2010-04-20 16:00:32
date last changed
2018-05-29 11:21:30
@article{d56fed6c-21fd-448f-a428-3cb4366dceec,
  abstract     = {Certain species of the fungal genus Trichoderma are potent mycoparasites and are used for biological control of fungal diseases on agricultural crops. In Trichoderma, whole-genome sequencing reveal between 20 and 36 different genes encoding chitinases, hydrolytic enzymes that are involved in the mycoparasitic attack. Sequences of Trichoderma chitinase genes chi18-5, chi18-13, chi18-15 and chi18-17, which all exhibit specific expression during mycoparasitism-related conditions, were determined from up to 13 different taxa and studied with regard to their evolutionary patterns. Two of them, chi18-13 and chi18-17, are members of the B1/B2 chitinase subgroup that have expanded significantly in paralog number in mycoparasitic Hypocrea atroviridis and H. virens. Chi18-13 contains two codons that evolve under positive selection and seven groups of co-evolving sites. Chi18-15 displays a unique codon-usage and contains five codons that evolve under positive selection and three groups of co-evolving sites. Regions of high amino acid variability are preferentially localized to substrate-or product side of the catalytic clefts. Differences in amino acid diversity/conservation patterns between different Trichoderma clades are observed. These observations show that Trichoderma chitinases chi18-13 and chi18-15 evolve in a manner consistent with rapid co-evolutionary interactions and identifies putative target regions involved in determining substrate-specificity.},
  author       = {Ihrmark, Katarina and Asmail, Nashwan and Ubhayasekera, Wimal and Melin, Petter and Stenlid, Jan and Karlsson, Magnus},
  issn         = {1176-9343},
  keyword      = {chitinase,mycoparasitism,protein evolution,Trichoderma},
  language     = {eng},
  pages        = {1--25},
  publisher    = {Libertas Academica},
  series       = {Evolutionary Bioinformatics},
  title        = {Comparative Molecular Evolution of Trichoderma Chitinases in Response to Mycoparasitic Interactions},
  volume       = {6},
  year         = {2010},
}