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Sulfite biosensor based on osmium redox polymer wired sulfite oxidase

Spricigo, Roberto; Richter, Claudia; Leimkuehler, Silke; Gorton, Lo LU ; Scheller, Frieder W. and Wollenberger, Ulla (2010) In Colloids and Surfaces A: Physicochemical and Engineering Aspects 354(1-3). p.314-319
Abstract
A biosensor, based on a redoxactive osmium polymer and sulfite oxidase on screen-printed electrodes, is presented here as a promising method for the detection of sulfite. A catalytic oxidative current was generated when a sample containing sulfite was pumped over the carbon screen-printed electrode modified with osmium redox polymer wired sulfite oxidase. A stationary value was reached after approximately 50 s and a complete measurement lasted no more than 3 min. The electrode polarized at -0.1 V (vs. Ag vertical bar AgCl 1M KCl) permits minimizing the influence of interfering substances, since these compounds can be unspecific oxidized at higher potentials. Because of the good stability of the protein film on the electrode surface, a well... (More)
A biosensor, based on a redoxactive osmium polymer and sulfite oxidase on screen-printed electrodes, is presented here as a promising method for the detection of sulfite. A catalytic oxidative current was generated when a sample containing sulfite was pumped over the carbon screen-printed electrode modified with osmium redox polymer wired sulfite oxidase. A stationary value was reached after approximately 50 s and a complete measurement lasted no more than 3 min. The electrode polarized at -0.1 V (vs. Ag vertical bar AgCl 1M KCl) permits minimizing the influence of interfering substances, since these compounds can be unspecific oxidized at higher potentials. Because of the good stability of the protein film on the electrode surface, a well functioning biosensor-flow system was possible to construct. The working stability and reproducibility were further enhanced by the addition of bovine serum albumin generating a more long-term stable and biocompatible protein environment. The optimized biosensor showed a stable signal for more than a week of operation and a coefficient of variation of 4.8% for 12 successive measurements. The lower limit of detection of the sensor was 0.5 mu M sulfite and the response was linear until 100 mu M. The high sensitivity permitted a 1:500 dilution of wine samples. The immobilization procedure and the operational conditions granted minimized interferences. Additionally, repeating the immobilization procedure to form several layers of wired SO further increased the sensitivity of such a sensor. Finally. the applicability of the developed sulfite biosensor was tested on real samples, such as white and red wines. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
redox polymer, Osmium, Surface immobilization, Biosensor, Sulfite, Sulfite oxidase
in
Colloids and Surfaces A: Physicochemical and Engineering Aspects
volume
354
issue
1-3
pages
314 - 319
publisher
Elsevier
external identifiers
  • wos:000275351300043
  • scopus:73049117821
ISSN
0927-7757
DOI
10.1016/j.colsurfa.2009.09.001
language
English
LU publication?
yes
id
ceec584a-bb37-4edc-96c4-25e960e23af0 (old id 1589111)
date added to LUP
2010-04-20 13:50:01
date last changed
2018-05-29 09:44:28
@article{ceec584a-bb37-4edc-96c4-25e960e23af0,
  abstract     = {A biosensor, based on a redoxactive osmium polymer and sulfite oxidase on screen-printed electrodes, is presented here as a promising method for the detection of sulfite. A catalytic oxidative current was generated when a sample containing sulfite was pumped over the carbon screen-printed electrode modified with osmium redox polymer wired sulfite oxidase. A stationary value was reached after approximately 50 s and a complete measurement lasted no more than 3 min. The electrode polarized at -0.1 V (vs. Ag vertical bar AgCl 1M KCl) permits minimizing the influence of interfering substances, since these compounds can be unspecific oxidized at higher potentials. Because of the good stability of the protein film on the electrode surface, a well functioning biosensor-flow system was possible to construct. The working stability and reproducibility were further enhanced by the addition of bovine serum albumin generating a more long-term stable and biocompatible protein environment. The optimized biosensor showed a stable signal for more than a week of operation and a coefficient of variation of 4.8% for 12 successive measurements. The lower limit of detection of the sensor was 0.5 mu M sulfite and the response was linear until 100 mu M. The high sensitivity permitted a 1:500 dilution of wine samples. The immobilization procedure and the operational conditions granted minimized interferences. Additionally, repeating the immobilization procedure to form several layers of wired SO further increased the sensitivity of such a sensor. Finally. the applicability of the developed sulfite biosensor was tested on real samples, such as white and red wines. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Spricigo, Roberto and Richter, Claudia and Leimkuehler, Silke and Gorton, Lo and Scheller, Frieder W. and Wollenberger, Ulla},
  issn         = {0927-7757},
  keyword      = {redox polymer,Osmium,Surface immobilization,Biosensor,Sulfite,Sulfite oxidase},
  language     = {eng},
  number       = {1-3},
  pages        = {314--319},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  title        = {Sulfite biosensor based on osmium redox polymer wired sulfite oxidase},
  url          = {http://dx.doi.org/10.1016/j.colsurfa.2009.09.001},
  volume       = {354},
  year         = {2010},
}