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Molecular phylogenetic and morphological analysis of haemosporidian parasites (Haemosporida) in a naturally infected European songbird, the blackcap Sylvia atricapilla, with description of Haemoproteus pallidulus sp nov.

Krizanauskiene, Asta; Perez-Tris, Javier; Palinauskas, Vaidas; Hellgren, Olof; Bensch, Staffan LU and Valkiunas, Gediminas (2010) In Parasitology 137(2). p.217-227
Abstract
The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and microscopy data for detection of pigment-forming haemosporidians of the genera Haemoproteus and Plasmodium. Blood samples from 498 blackcaps were collected at 7 different sites in Europe and investigated for these parasites by polymerase chain reaction (PCR)-based techniques and microscopic examination. In all, 56% of the birds were infected by at least 1 out of 25 distinct mitochondrial cytochrome b (cyt b) gene lineages of these... (More)
The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and microscopy data for detection of pigment-forming haemosporidians of the genera Haemoproteus and Plasmodium. Blood samples from 498 blackcaps were collected at 7 different sites in Europe and investigated for these parasites by polymerase chain reaction (PCR)-based techniques and microscopic examination. In all, 56% of the birds were infected by at least 1 out of 25 distinct mitochondrial cytochrome b (cyt b) gene lineages of these haemosporidians. It is concluded that the blackcap is infected not only with blackcap specific haemosporidians, but also with Haemoproteus majoris, which is a host generalist and common in birds belonging to the Paridae. Haemoproteus pallidulus sp. nov. is described based on morphology of its blood stages and segments of the cyt b and dihydrofolate reductase/thymidylate synthase (DHFR-TS) genes. This study provides evidence that genetic diversity of haemosporidian parasites might be positively correlated with migratory strategies of their avian hosts; it also contributes to the value of both microscopy and molecular diagnostics of avian blood parasites. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sylvia atricapilla, avian haemosporidians, Haemoproteus, Plasmodium, PCR, microscopy, mitochondrial DNA, phylogeography
in
Parasitology
volume
137
issue
2
pages
217 - 227
publisher
Cambridge University Press
external identifiers
  • wos:000275225600003
  • scopus:77949654080
ISSN
1469-8161
DOI
10.1017/S0031182009991235
project
BECC
language
English
LU publication?
yes
id
85071146-e399-48ed-8743-9ef6878173bc (old id 1589405)
date added to LUP
2010-04-20 11:17:32
date last changed
2018-06-03 03:13:34
@article{85071146-e399-48ed-8743-9ef6878173bc,
  abstract     = {The blackcap (Sylvia atricapilla) is a common Palearctic migratory warbler, and haemosporidian parasites are common in this species. However, genetic and phenotypic diversity of haemosporidians in warblers has been insufficiently investigated and poorly linked. We addressed this issue by combining molecular and microscopy data for detection of pigment-forming haemosporidians of the genera Haemoproteus and Plasmodium. Blood samples from 498 blackcaps were collected at 7 different sites in Europe and investigated for these parasites by polymerase chain reaction (PCR)-based techniques and microscopic examination. In all, 56% of the birds were infected by at least 1 out of 25 distinct mitochondrial cytochrome b (cyt b) gene lineages of these haemosporidians. It is concluded that the blackcap is infected not only with blackcap specific haemosporidians, but also with Haemoproteus majoris, which is a host generalist and common in birds belonging to the Paridae. Haemoproteus pallidulus sp. nov. is described based on morphology of its blood stages and segments of the cyt b and dihydrofolate reductase/thymidylate synthase (DHFR-TS) genes. This study provides evidence that genetic diversity of haemosporidian parasites might be positively correlated with migratory strategies of their avian hosts; it also contributes to the value of both microscopy and molecular diagnostics of avian blood parasites.},
  author       = {Krizanauskiene, Asta and Perez-Tris, Javier and Palinauskas, Vaidas and Hellgren, Olof and Bensch, Staffan and Valkiunas, Gediminas},
  issn         = {1469-8161},
  keyword      = {Sylvia atricapilla,avian haemosporidians,Haemoproteus,Plasmodium,PCR,microscopy,mitochondrial DNA,phylogeography},
  language     = {eng},
  number       = {2},
  pages        = {217--227},
  publisher    = {Cambridge University Press},
  series       = {Parasitology},
  title        = {Molecular phylogenetic and morphological analysis of haemosporidian parasites (Haemosporida) in a naturally infected European songbird, the blackcap Sylvia atricapilla, with description of Haemoproteus pallidulus sp nov.},
  url          = {http://dx.doi.org/10.1017/S0031182009991235},
  volume       = {137},
  year         = {2010},
}