Competitive capacitive biosensing technique (CCBT): A novel technique for monitoring low molecular mass analytes using glucose assay as a model study.
(2010) In Analytical and Bioanalytical Chemistry 397. p.1217-1224- Abstract
- A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance... (More)
- A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance depends linearly on glucose concentration within the range from 1.0 x 10(-5) to 1.0 x 10(-1) M, corresponding to 1.8 microg ml(-1) to 18 mg ml(-1) in a logarithmic plot, with a detection limit of 1.0 x 10(-6) (0.18 microg ml(-1)) under optimized conditions. In addition, by modifying the molecular mass of the glucose polymer, amount of biorecognition element, and buffer composition, we were able to tune the analyte-sensing range. The developed technique has the benefits of expanded dynamic range, high sensitivity, and excellent reusability. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1595102
- author
- Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical and Bioanalytical Chemistry
- volume
- 397
- pages
- 1217 - 1224
- publisher
- Springer
- external identifiers
-
- wos:000277593200030
- pmid:20401723
- scopus:77953477424
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-010-3641-8
- language
- English
- LU publication?
- yes
- id
- 56f3c6cb-a411-406f-939c-d93d86a58921 (old id 1595102)
- date added to LUP
- 2016-04-01 09:51:13
- date last changed
- 2022-01-25 17:18:45
@article{56f3c6cb-a411-406f-939c-d93d86a58921,
abstract = {{A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance depends linearly on glucose concentration within the range from 1.0 x 10(-5) to 1.0 x 10(-1) M, corresponding to 1.8 microg ml(-1) to 18 mg ml(-1) in a logarithmic plot, with a detection limit of 1.0 x 10(-6) (0.18 microg ml(-1)) under optimized conditions. In addition, by modifying the molecular mass of the glucose polymer, amount of biorecognition element, and buffer composition, we were able to tune the analyte-sensing range. The developed technique has the benefits of expanded dynamic range, high sensitivity, and excellent reusability.}},
author = {{Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo}},
issn = {{1618-2642}},
language = {{eng}},
pages = {{1217--1224}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Competitive capacitive biosensing technique (CCBT): A novel technique for monitoring low molecular mass analytes using glucose assay as a model study.}},
url = {{http://dx.doi.org/10.1007/s00216-010-3641-8}},
doi = {{10.1007/s00216-010-3641-8}},
volume = {{397}},
year = {{2010}},
}