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Competitive capacitive biosensing technique (CCBT): A novel technique for monitoring low molecular mass analytes using glucose assay as a model study.

Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU (2010) In Analytical and Bioanalytical Chemistry 397. p.1217-1224
Abstract
A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance... (More)
A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance depends linearly on glucose concentration within the range from 1.0 x 10(-5) to 1.0 x 10(-1) M, corresponding to 1.8 microg ml(-1) to 18 mg ml(-1) in a logarithmic plot, with a detection limit of 1.0 x 10(-6) (0.18 microg ml(-1)) under optimized conditions. In addition, by modifying the molecular mass of the glucose polymer, amount of biorecognition element, and buffer composition, we were able to tune the analyte-sensing range. The developed technique has the benefits of expanded dynamic range, high sensitivity, and excellent reusability. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
397
pages
1217 - 1224
publisher
Springer
external identifiers
  • wos:000277593200030
  • pmid:20401723
  • scopus:77953477424
ISSN
1618-2642
DOI
10.1007/s00216-010-3641-8
language
English
LU publication?
yes
id
56f3c6cb-a411-406f-939c-d93d86a58921 (old id 1595102)
date added to LUP
2010-05-06 14:36:19
date last changed
2018-05-29 11:59:34
@article{56f3c6cb-a411-406f-939c-d93d86a58921,
  abstract     = {A novel technique for monitoring of low molecular mass analytes using a flow-injection capacitive biosensor is presented. The method is based on the ability of a small molecular mass analyte to displace a large analyte-carrier conjugate from the binding sites of an immobilized biorecognition element with weak affinity to both compounds. A model study was performed on glucose as the small molecular mass analyte. In the absence of glucose, binding of a glucose polymer or a glycoconjugate to concanavalin A results in a capacitance decrease. Upon introduction of glucose, it displaces a part of the bound glucose polymer or glycoconjugate leading to a partial restoration of capacitance. Experimental results show that the change in capacitance depends linearly on glucose concentration within the range from 1.0 x 10(-5) to 1.0 x 10(-1) M, corresponding to 1.8 microg ml(-1) to 18 mg ml(-1) in a logarithmic plot, with a detection limit of 1.0 x 10(-6) (0.18 microg ml(-1)) under optimized conditions. In addition, by modifying the molecular mass of the glucose polymer, amount of biorecognition element, and buffer composition, we were able to tune the analyte-sensing range. The developed technique has the benefits of expanded dynamic range, high sensitivity, and excellent reusability.},
  author       = {Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo},
  issn         = {1618-2642},
  language     = {eng},
  pages        = {1217--1224},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Competitive capacitive biosensing technique (CCBT): A novel technique for monitoring low molecular mass analytes using glucose assay as a model study.},
  url          = {http://dx.doi.org/10.1007/s00216-010-3641-8},
  volume       = {397},
  year         = {2010},
}