Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis
(2014) 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 p.2540-2541- Abstract
In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/15d346e8-48f8-4e50-a010-25fc1c3dd625
- author
- Cushing, Kevin W. LU and Undvall, Eva LU
- organization
- publishing date
- 2014
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustophoresis, Circulating tumor cells, Elastomeric particles, Label-free enrichment, Negative selection
- host publication
- 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
- conference location
- San Antonio, United States
- conference dates
- 2014-10-26 - 2014-10-30
- external identifiers
-
- scopus:84941712257
- ISBN
- 9780979806476
- 9781634396974
- language
- English
- LU publication?
- yes
- id
- 15d346e8-48f8-4e50-a010-25fc1c3dd625
- date added to LUP
- 2017-12-08 10:47:32
- date last changed
- 2025-01-08 02:39:52
@inproceedings{15d346e8-48f8-4e50-a010-25fc1c3dd625, abstract = {{<p>In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip.</p>}}, author = {{Cushing, Kevin W. and Undvall, Eva}}, booktitle = {{18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014}}, isbn = {{9780979806476}}, keywords = {{Acoustophoresis; Circulating tumor cells; Elastomeric particles; Label-free enrichment; Negative selection}}, language = {{eng}}, pages = {{2540--2541}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis}}, year = {{2014}}, }