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Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis

Cushing, Kevin W. LU and Undvall, Eva LU (2014) 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 p.2540-2541
Abstract

In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustophoresis, Circulating tumor cells, Elastomeric particles, Label-free enrichment, Negative selection
host publication
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
conference location
San Antonio, United States
conference dates
2014-10-26 - 2014-10-30
external identifiers
  • scopus:84941712257
ISBN
9780979806476
9781634396974
language
English
LU publication?
yes
id
15d346e8-48f8-4e50-a010-25fc1c3dd625
date added to LUP
2017-12-08 10:47:32
date last changed
2019-02-20 11:00:06
@inproceedings{15d346e8-48f8-4e50-a010-25fc1c3dd625,
  abstract     = {<p>In an effort to bioengineer elastomeric particles (EPs), with negative acoustic contrast factor, to capture cells, we have successfully functionalized EPs with anti-human CD45 antibodies that enable capturing of WBCs from human RBC lysed blood samples. We demonstrate that the negative selection of WBCs with EPs and acoustophoresis can be employed to reduce WBC contamination while enriching label-free prostate cancer cells (DU145) within the central fraction of our acoustophoresis chip.</p>},
  author       = {Cushing, Kevin W. and Undvall, Eva},
  isbn         = {9780979806476},
  keyword      = {Acoustophoresis,Circulating tumor cells,Elastomeric particles,Label-free enrichment,Negative selection},
  language     = {eng},
  location     = {San Antonio, United States},
  pages        = {2540--2541},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Label-free enrichment of cancer cells using negative selection (depletion) of wbcs with elastomeric negative acoustic contrast particles and acoustophoresis},
  year         = {2014},
}