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The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking

Englund, Mikael C. O.; Caisander, Gunilla; Noaksson, Karin; Emanuelsson, Katarina; Lundin, Kersti; Bergh, Christina; Hansson, Charles; Semb, Henrik LU ; Strehl, Raimund and Hyllner, Johan (2010) In In Vitro Cellular & Developmental Biology - Animal 46(3-4). p.217-230
Abstract
This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for... (More)
This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for undifferentiated hES cells. In addition to the traditional manual dissection approach of propagating hES cells, we here also report on the successful approaches of feeder-free cultures as well as single cell cultures based on enzymatic digestion. All culture systems used as reported here have maintained the hES cells in a karyotypical normal and pluripotent state. These systems also have the advantage of being the principal springboards for further scale up of cultures for industrial or clinical applications that would require vastly more cells that can be produced by mechanical means. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell banking, Characterisation, Pluripotency, Single cells, Feeder-free, Xeno-free, hES cells, Derivation
in
In Vitro Cellular & Developmental Biology - Animal
volume
46
issue
3-4
pages
217 - 230
publisher
Springer
external identifiers
  • wos:000276768700010
  • scopus:77952096311
ISSN
1071-2690
DOI
10.1007/s11626-010-9289-z
language
English
LU publication?
yes
id
ab9c5dcf-aa38-459a-a5b2-358d22a779e6 (old id 1603037)
date added to LUP
2010-05-19 09:53:08
date last changed
2018-05-29 10:19:08
@article{ab9c5dcf-aa38-459a-a5b2-358d22a779e6,
  abstract     = {This report summarises our efforts in deriving, characterising and banking of 20 different human embryonic stem cell lines. We have derived a large number of human embryonic stem cell lines between 2001 and 2005. One of these cell lines was established under totally xeno-free culture conditions. In addition, several subclones have been established, including a karyoptypical normal clone from a trisomic mother line. A master cell banking system has been utilised in concert with an extensive characterisation programme, ensuring a supply of high quality pluripotent stem cells for further research and development. In this report we also present the first data on a proprietary novel antibody, hES-Cellect, that exhibits high specificity for undifferentiated hES cells. In addition to the traditional manual dissection approach of propagating hES cells, we here also report on the successful approaches of feeder-free cultures as well as single cell cultures based on enzymatic digestion. All culture systems used as reported here have maintained the hES cells in a karyotypical normal and pluripotent state. These systems also have the advantage of being the principal springboards for further scale up of cultures for industrial or clinical applications that would require vastly more cells that can be produced by mechanical means.},
  author       = {Englund, Mikael C. O. and Caisander, Gunilla and Noaksson, Karin and Emanuelsson, Katarina and Lundin, Kersti and Bergh, Christina and Hansson, Charles and Semb, Henrik and Strehl, Raimund and Hyllner, Johan},
  issn         = {1071-2690},
  keyword      = {Cell banking,Characterisation,Pluripotency,Single cells,Feeder-free,Xeno-free,hES cells,Derivation},
  language     = {eng},
  number       = {3-4},
  pages        = {217--230},
  publisher    = {Springer},
  series       = {In Vitro Cellular & Developmental Biology - Animal},
  title        = {The establishment of 20 different human embryonic stem cell lines and subclones; a report on derivation, culture, characterisation and banking},
  url          = {http://dx.doi.org/10.1007/s11626-010-9289-z},
  volume       = {46},
  year         = {2010},
}