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Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood

Derazshamshir, Ali ; Baydemir, Goezde ; Andac, Muege ; Say, Ridvan ; Galaev, Igor LU and Denizli, Adil (2010) In Macromolecular Chemistry and Physics 211(6). p.657-668
Abstract
A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption... (More)
A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hydrogels, hemoglobin depletion, affinity binding, cryogels, molecular, recognition
in
Macromolecular Chemistry and Physics
volume
211
issue
6
pages
657 - 668
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000276436300007
  • scopus:77949864264
ISSN
1521-3935
DOI
10.1002/macp.200900425
language
English
LU publication?
yes
id
0e2e5ea8-5981-459d-a2b3-64df18863d39 (old id 1603814)
date added to LUP
2016-04-01 10:49:02
date last changed
2022-03-12 17:22:57
@article{0e2e5ea8-5981-459d-a2b3-64df18863d39,
  abstract     = {{A new generation of molecularly imprinted macroporous hydrogels, so-called cryogels, were prepared for depletion of hemoglobin from human blood. Hb-PHEMAH cryogels were prepared with gel fraction yields up to 90%, and its morphology and porosity were characterized by FTIR, SEM, swelling studies, flow dynamics and surface area measurements. Selective binding experiments were performed in the presence of competitive PHEMAH cryogel exhibited a high binding capacity and selectivity for Hb in the presence of Myb and BSA. The selectivity of Hb-PHEMAH cryogel for Hb was confirmed by HPLC. Hb depletion from blood hemolysate was also studied using SDS-PAGE. Hb-PHE-MAH cryogel can be reused many times with no apparent decrease in Hb adsorption capacity.}},
  author       = {{Derazshamshir, Ali and Baydemir, Goezde and Andac, Muege and Say, Ridvan and Galaev, Igor and Denizli, Adil}},
  issn         = {{1521-3935}},
  keywords     = {{hydrogels; hemoglobin depletion; affinity binding; cryogels; molecular; recognition}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{657--668}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Macromolecular Chemistry and Physics}},
  title        = {{Molecularly Imprinted PHEMA-Based Cryogel for Depletion of Hemoglobin from Human Blood}},
  url          = {{http://dx.doi.org/10.1002/macp.200900425}},
  doi          = {{10.1002/macp.200900425}},
  volume       = {{211}},
  year         = {{2010}},
}