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Direct electrochemistry and bioelectrocatalysis of a class II non-symbiotic plant haemoglobin immobilised on screen-printed carbon electrodes.

Chekin, Fereshteh LU ; Leiva, Nélida LU ; Raoof, Jahan Bakhsh; Gorton, Lo LU and Bülow, Leif LU (2010) In Analytical and Bioanalytical Chemistry 398. p.1643-1649
Abstract
In this study, direct electron transfer (ET) has been achieved between an immobilised non-symbiotic plant haemoglobin class II from Beta vulgaris (nsBvHb2) and three different screen-printed carbon electrodes based on graphite (SPCE), multi-walled carbon nanotubes (MWCNT-SPCE), and single-walled carbon nanotubes (SWCNT-SPCE) without the aid of any electron mediator. The nsBvHb2 modified electrodes were studied with cyclic voltammetry (CV) and also when placed in a wall-jet flow through cell for their electrocatalytic properties for reduction of H(2)O(2). The immobilised nsBvHb2 displayed a couple of stable and well-defined redox peaks with a formal potential (E degrees ') of -33.5 mV (vs. Ag|AgCl|3 M KCl) at pH 7.4. The ET rate constant of... (More)
In this study, direct electron transfer (ET) has been achieved between an immobilised non-symbiotic plant haemoglobin class II from Beta vulgaris (nsBvHb2) and three different screen-printed carbon electrodes based on graphite (SPCE), multi-walled carbon nanotubes (MWCNT-SPCE), and single-walled carbon nanotubes (SWCNT-SPCE) without the aid of any electron mediator. The nsBvHb2 modified electrodes were studied with cyclic voltammetry (CV) and also when placed in a wall-jet flow through cell for their electrocatalytic properties for reduction of H(2)O(2). The immobilised nsBvHb2 displayed a couple of stable and well-defined redox peaks with a formal potential (E degrees ') of -33.5 mV (vs. Ag|AgCl|3 M KCl) at pH 7.4. The ET rate constant of nsBvHb2, k (s), was also determined at the surface of the three types of electrodes in phosphate buffer solution pH 7.4, and was found to be 0.50 s(-1) on SPCE, 2.78 s(-1) on MWCNT-SPCE and 4.06 s(-1) on SWCNT-SPCE, respectively. The average surface coverage of electrochemically active nsBvHb2 immobilised on the SPCEs, MWCNT-SPCEs and SWCNT-SPCEs obtained was 2.85 x 10(-10) mol cm(-2), 4.13 x 10(-10) mol cm(-2) and 5.20 x 10(-10) mol cm(-2). During the experiments the immobilised nsBvHb2 was stable and kept its electrochemical and catalytic activities. The nsBvHb2 modified electrodes also displayed an excellent response to the reduction of hydrogen peroxide (H(2)O(2)) with a linear detection range from 1 muM to 1000 muM on the surface of SPCEs, from 0.5 muM to 1000 muM on MWCNT-SPCEs, and from 0.1 muM to 1000 muM on SWCNT-SPCEs. The lower limit of detection was 0.8 muM, 0.4 muM and 0.1 muM at 3sigma at the SPCEs, the MWCNT-SPCEs, and the SWCNT-SPCEs, respectively, and the apparent Michaelis-Menten constant, [Formula: see text], for the H(2)O(2) sensors was estimated to be 0.32 mM , 0.29 mM and 0.27 mM, respectively. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
398
pages
1643 - 1649
publisher
Springer
external identifiers
  • wos:000282090000010
  • pmid:20506016
  • scopus:77957846484
ISSN
1618-2642
DOI
10.1007/s00216-010-3800-y
language
English
LU publication?
yes
id
289b2fba-a64b-4bb7-b80c-9c187325217b (old id 1609862)
date added to LUP
2010-06-10 17:07:28
date last changed
2018-05-29 10:04:48
@article{289b2fba-a64b-4bb7-b80c-9c187325217b,
  abstract     = {In this study, direct electron transfer (ET) has been achieved between an immobilised non-symbiotic plant haemoglobin class II from Beta vulgaris (nsBvHb2) and three different screen-printed carbon electrodes based on graphite (SPCE), multi-walled carbon nanotubes (MWCNT-SPCE), and single-walled carbon nanotubes (SWCNT-SPCE) without the aid of any electron mediator. The nsBvHb2 modified electrodes were studied with cyclic voltammetry (CV) and also when placed in a wall-jet flow through cell for their electrocatalytic properties for reduction of H(2)O(2). The immobilised nsBvHb2 displayed a couple of stable and well-defined redox peaks with a formal potential (E degrees ') of -33.5 mV (vs. Ag|AgCl|3 M KCl) at pH 7.4. The ET rate constant of nsBvHb2, k (s), was also determined at the surface of the three types of electrodes in phosphate buffer solution pH 7.4, and was found to be 0.50 s(-1) on SPCE, 2.78 s(-1) on MWCNT-SPCE and 4.06 s(-1) on SWCNT-SPCE, respectively. The average surface coverage of electrochemically active nsBvHb2 immobilised on the SPCEs, MWCNT-SPCEs and SWCNT-SPCEs obtained was 2.85 x 10(-10) mol cm(-2), 4.13 x 10(-10) mol cm(-2) and 5.20 x 10(-10) mol cm(-2). During the experiments the immobilised nsBvHb2 was stable and kept its electrochemical and catalytic activities. The nsBvHb2 modified electrodes also displayed an excellent response to the reduction of hydrogen peroxide (H(2)O(2)) with a linear detection range from 1 muM to 1000 muM on the surface of SPCEs, from 0.5 muM to 1000 muM on MWCNT-SPCEs, and from 0.1 muM to 1000 muM on SWCNT-SPCEs. The lower limit of detection was 0.8 muM, 0.4 muM and 0.1 muM at 3sigma at the SPCEs, the MWCNT-SPCEs, and the SWCNT-SPCEs, respectively, and the apparent Michaelis-Menten constant, [Formula: see text], for the H(2)O(2) sensors was estimated to be 0.32 mM , 0.29 mM and 0.27 mM, respectively.},
  author       = {Chekin, Fereshteh and Leiva, Nélida and Raoof, Jahan Bakhsh and Gorton, Lo and Bülow, Leif},
  issn         = {1618-2642},
  language     = {eng},
  pages        = {1643--1649},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Direct electrochemistry and bioelectrocatalysis of a class II non-symbiotic plant haemoglobin immobilised on screen-printed carbon electrodes.},
  url          = {http://dx.doi.org/10.1007/s00216-010-3800-y},
  volume       = {398},
  year         = {2010},
}