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Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.

Broos, Sissela LU ; Lundberg, Kristina LU ; Akagi, Takami; Kadowaki, Koji LU ; Akashi, Mitsuru; Greiff, Lennart LU ; Borrebaeck, Carl LU and Lindstedt, Malin LU (2010) In Vaccine 28. p.5075-5085
Abstract
Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and... (More)
Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vaccine
volume
28
pages
5075 - 5085
publisher
Elsevier
external identifiers
  • pmid:20478343
  • wos:000280659600031
  • scopus:77954760985
ISSN
1873-2518
DOI
10.1016/j.vaccine.2010.05.004
language
English
LU publication?
yes
id
82b7bcc7-a99a-4f98-9ca9-2db85beefbbe (old id 1610149)
date added to LUP
2010-06-02 13:59:27
date last changed
2018-05-29 12:14:55
@article{82b7bcc7-a99a-4f98-9ca9-2db85beefbbe,
  abstract     = {Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy.},
  author       = {Broos, Sissela and Lundberg, Kristina and Akagi, Takami and Kadowaki, Koji and Akashi, Mitsuru and Greiff, Lennart and Borrebaeck, Carl and Lindstedt, Malin},
  issn         = {1873-2518},
  language     = {eng},
  pages        = {5075--5085},
  publisher    = {Elsevier},
  series       = {Vaccine},
  title        = {Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.},
  url          = {http://dx.doi.org/10.1016/j.vaccine.2010.05.004},
  volume       = {28},
  year         = {2010},
}