Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.
Broos, Sissela LU ; Lundberg, Kristina LU ; Akagi, Takami ; Kadowaki, Koji LU ; Akashi, Mitsuru ; Greiff, Lennart LU ; Borrebaeck, Carl LU and Lindstedt, Malin LU
(2010)
In Vaccine
28.
p.5075-5085
- Abstract
- Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and... (More)
- Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1610149
- author
- Broos, Sissela
LU
; Lundberg, Kristina
LU
; Akagi, Takami
; Kadowaki, Koji
LU
; Akashi, Mitsuru
; Greiff, Lennart
LU
; Borrebaeck, Carl
LU
and Lindstedt, Malin
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Vaccine
- volume
- 28
- pages
- 5075 - 5085
- publisher
- Elsevier
- external identifiers
-
- pmid:20478343
- wos:000280659600031
- scopus:77954760985
- pmid:20478343
- ISSN
- 1873-2518
- DOI
- 10.1016/j.vaccine.2010.05.004
- language
- English
- LU publication?
- yes
- id
- 82b7bcc7-a99a-4f98-9ca9-2db85beefbbe (old id 1610149)
- date added to LUP
- 2016-04-01 11:03:37
- date last changed
- 2024-07-29 11:59:21
@article{82b7bcc7-a99a-4f98-9ca9-2db85beefbbe,
abstract = {{Novel adjuvants and antigen-delivery systems with immunomodulatory properties that shift the allergenic Th2 response towards a Th1 or regulatory T cell response are desired for allergen-specific immunotherapy. This study demonstrates that 200-nm sized biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) are activators of human monocyte-derived dendritic cells (MoDCs). gamma-PGA NPs are efficiently internalized by immature MoDCs and strongly stimulate production of chemokines and inflammatory cytokines as well as up-regulation of co-stimulatory molecules and immunomodulatory mediators involved in efficient T cell priming. Furthermore, MoDCs from allergic subjects stimulated in vitro with a mixture of gamma-PGA NPs and extract of grass pollen allergen Phleum pratense (Phl p) augment allergen-specific IL-10 production and proliferation of autologous CD4(+) memory T cells. Thus, gamma-PGA NPs are promising as sophisticated adjuvants and allergen-delivery systems in allergen-specific immunotherapy.}},
author = {{Broos, Sissela and Lundberg, Kristina and Akagi, Takami and Kadowaki, Koji and Akashi, Mitsuru and Greiff, Lennart and Borrebaeck, Carl and Lindstedt, Malin}},
issn = {{1873-2518}},
language = {{eng}},
pages = {{5075--5085}},
publisher = {{Elsevier}},
series = {{Vaccine}},
title = {{Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells: Implications for specific immunotherapy.}},
url = {{http://dx.doi.org/10.1016/j.vaccine.2010.05.004}},
doi = {{10.1016/j.vaccine.2010.05.004}},
volume = {{28}},
year = {{2010}},
}