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A study of glycoprotein-lectin interactions using quartz crystal microbalance.

Yakovleva, Maria E; Safina, Gulnara LU and Danielsson, Bengt LU (2010) In Analytica Chimica Acta 668(1). p.80-85
Abstract
A study of biospecific interactions between lectins and glycoproteins using a quartz crystal microbalance biosensor with dissipation monitoring (QCM-D) was reported. Four lectins were covalently immobilised on the thiol-modified gold electrode of the QCM chips in order to obtain sensing surfaces. The frequency shift served as analytical signal and the dissipation shift provided additional information about the viscoelastic properties of the glycoprotein-lectin complex formed on the surface of the QCM chip. The working conditions of the assay were optimised. The interaction between different lectins and glycoproteins was characterised by specific frequency shifts and each glycoprotein displayed its own unique lectin-binding pattern. This... (More)
A study of biospecific interactions between lectins and glycoproteins using a quartz crystal microbalance biosensor with dissipation monitoring (QCM-D) was reported. Four lectins were covalently immobilised on the thiol-modified gold electrode of the QCM chips in order to obtain sensing surfaces. The frequency shift served as analytical signal and the dissipation shift provided additional information about the viscoelastic properties of the glycoprotein-lectin complex formed on the surface of the QCM chip. The working conditions of the assay were optimised. The interaction between different lectins and glycoproteins was characterised by specific frequency shifts and each glycoprotein displayed its own unique lectin-binding pattern. This lectin pattern can serve as a finger print for the discrimination between various glycoproteins. The biosensor enabled quantitative determination of glycoproteins in the concentration range of 50 microg mL(-1) to 1 mg mL(-1) with good linearity and R.S.D. of less than 6.0%. An additional advantage of the proposed biosensor was the possibility to re-use the same lectin surfaces during a long period of time (2 month) without changes in analytical response. This was experimentally achieved by the application of a proper regeneration solution (10 mM glycine-HCl, pH 2.5). The lectin-based quartz crystal microbalance technique is suitable both for rapid screening and for quantitative assay of serum glycoproteins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
668
issue
1
pages
80 - 85
publisher
Elsevier
external identifiers
  • wos:000278306800014
  • pmid:20457306
  • scopus:77952600756
ISSN
1873-4324
DOI
10.1016/j.aca.2009.12.004
language
English
LU publication?
yes
id
c860b04b-f061-4c17-ac5d-225fab9fb438 (old id 1610364)
date added to LUP
2010-06-02 12:55:49
date last changed
2018-05-29 11:41:40
@article{c860b04b-f061-4c17-ac5d-225fab9fb438,
  abstract     = {A study of biospecific interactions between lectins and glycoproteins using a quartz crystal microbalance biosensor with dissipation monitoring (QCM-D) was reported. Four lectins were covalently immobilised on the thiol-modified gold electrode of the QCM chips in order to obtain sensing surfaces. The frequency shift served as analytical signal and the dissipation shift provided additional information about the viscoelastic properties of the glycoprotein-lectin complex formed on the surface of the QCM chip. The working conditions of the assay were optimised. The interaction between different lectins and glycoproteins was characterised by specific frequency shifts and each glycoprotein displayed its own unique lectin-binding pattern. This lectin pattern can serve as a finger print for the discrimination between various glycoproteins. The biosensor enabled quantitative determination of glycoproteins in the concentration range of 50 microg mL(-1) to 1 mg mL(-1) with good linearity and R.S.D. of less than 6.0%. An additional advantage of the proposed biosensor was the possibility to re-use the same lectin surfaces during a long period of time (2 month) without changes in analytical response. This was experimentally achieved by the application of a proper regeneration solution (10 mM glycine-HCl, pH 2.5). The lectin-based quartz crystal microbalance technique is suitable both for rapid screening and for quantitative assay of serum glycoproteins.},
  author       = {Yakovleva, Maria E and Safina, Gulnara and Danielsson, Bengt},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1},
  pages        = {80--85},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {A study of glycoprotein-lectin interactions using quartz crystal microbalance.},
  url          = {http://dx.doi.org/10.1016/j.aca.2009.12.004},
  volume       = {668},
  year         = {2010},
}