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Fructose-selective calorimetric biosensor in flow injection analysis.

Bhand, Sunil LU ; Srimathi, Soundararajan LU ; Wärnmark, Ioana LU ; Milea, Jaqueline Simona; Dey, Estera LU ; Yakovleva, Maria LU and Danielsson, Bengt LU (2010) In Analytica Chimica Acta 668(1). p.13-18
Abstract
A highly selective, interference free biosensor for the measurement of fructose in real syrup samples was developed. The assay is based on the phosphorylation of D(-)fructose to fructose-6-phosphate by hexokinase and subsequent conversion of fructose-6-phosphate to fructose-1,6-biphosphate by fructose-6-phosphate-kinase. The heat liberated in the second reaction is monitored using an enzyme thermistor. The major advantages of this biosensor are rapid and selective measurement of fructose without the need to eliminate glucose and inexpensive FIA-based, mediator-free calorimetric measurement suitable for regular fructose analysis. This method was optimised for parameters, such as pH, ionic strength, interference, operational stability and... (More)
A highly selective, interference free biosensor for the measurement of fructose in real syrup samples was developed. The assay is based on the phosphorylation of D(-)fructose to fructose-6-phosphate by hexokinase and subsequent conversion of fructose-6-phosphate to fructose-1,6-biphosphate by fructose-6-phosphate-kinase. The heat liberated in the second reaction is monitored using an enzyme thermistor. The major advantages of this biosensor are rapid and selective measurement of fructose without the need to eliminate glucose and inexpensive FIA-based, mediator-free calorimetric measurement suitable for regular fructose analysis. This method was optimised for parameters, such as pH, ionic strength, interference, operational stability and shelf life. Good and reproducible linearity (0.5-6.0 mM) with a detection limit of 0.12 mM was obtained. Fructose determination in commercial syrup samples and spiked samples confirmed the reliability of this set-up and technique. The biosensor gave reproducible results with good overall stability for continuous measurements over a period of three months besides a useful shelf life of six months. The method could be used for routine fructose monitoring in food samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
668
issue
1
pages
13 - 18
publisher
Elsevier
external identifiers
  • wos:000278306800004
  • pmid:20457296
  • scopus:77952674754
ISSN
1873-4324
DOI
10.1016/j.aca.2010.01.020
language
English
LU publication?
yes
id
21cbb840-5b0f-4d47-ae32-4ac4f6d1b828 (old id 1610370)
date added to LUP
2010-06-02 13:00:24
date last changed
2018-05-29 11:45:48
@article{21cbb840-5b0f-4d47-ae32-4ac4f6d1b828,
  abstract     = {A highly selective, interference free biosensor for the measurement of fructose in real syrup samples was developed. The assay is based on the phosphorylation of D(-)fructose to fructose-6-phosphate by hexokinase and subsequent conversion of fructose-6-phosphate to fructose-1,6-biphosphate by fructose-6-phosphate-kinase. The heat liberated in the second reaction is monitored using an enzyme thermistor. The major advantages of this biosensor are rapid and selective measurement of fructose without the need to eliminate glucose and inexpensive FIA-based, mediator-free calorimetric measurement suitable for regular fructose analysis. This method was optimised for parameters, such as pH, ionic strength, interference, operational stability and shelf life. Good and reproducible linearity (0.5-6.0 mM) with a detection limit of 0.12 mM was obtained. Fructose determination in commercial syrup samples and spiked samples confirmed the reliability of this set-up and technique. The biosensor gave reproducible results with good overall stability for continuous measurements over a period of three months besides a useful shelf life of six months. The method could be used for routine fructose monitoring in food samples.},
  author       = {Bhand, Sunil and Srimathi, Soundararajan and Wärnmark, Ioana and Milea, Jaqueline Simona and Dey, Estera and Yakovleva, Maria and Danielsson, Bengt},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1},
  pages        = {13--18},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Fructose-selective calorimetric biosensor in flow injection analysis.},
  url          = {http://dx.doi.org/10.1016/j.aca.2010.01.020},
  volume       = {668},
  year         = {2010},
}