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Soil bacterial and fungal communities across a pH gradient in an arable soil.

Rousk, Johannes LU ; Bååth, Erland LU ; Brookes, Philip C; Lauber, Christian L; Lozupone, Catherine; Caporaso, J Gregory; Knight, Rob and Fierer, Noah (2010) In The Isme Journal 4. p.1340-1351
Abstract
Soils collected across a long-term liming experiment (pH 4.0-8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria. We hypothesized that bacterial communities would be more strongly influenced by pH than fungal communities. To determine the relative abundance of bacteria and fungi, we used quantitative PCR (qPCR), and to analyze the composition and diversity of the bacterial and fungal communities, we used a bar-coded pyrosequencing technique. Both the relative abundance and diversity of bacteria were positively related to pH, the latter nearly doubling between pH 4 and 8. In... (More)
Soils collected across a long-term liming experiment (pH 4.0-8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria. We hypothesized that bacterial communities would be more strongly influenced by pH than fungal communities. To determine the relative abundance of bacteria and fungi, we used quantitative PCR (qPCR), and to analyze the composition and diversity of the bacterial and fungal communities, we used a bar-coded pyrosequencing technique. Both the relative abundance and diversity of bacteria were positively related to pH, the latter nearly doubling between pH 4 and 8. In contrast, the relative abundance of fungi was unaffected by pH and fungal diversity was only weakly related with pH. The composition of the bacterial communities was closely defined by soil pH; there was as much variability in bacterial community composition across the 180-m distance of this liming experiment as across soils collected from a wide range of biomes in North and South America, emphasizing the dominance of pH in structuring bacterial communities. The apparent direct influence of pH on bacterial community composition is probably due to the narrow pH ranges for optimal growth of bacteria. Fungal community composition was less strongly affected by pH, which is consistent with pure culture studies, demonstrating that fungi generally exhibit wider pH ranges for optimal growth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Isme Journal
volume
4
pages
1340 - 1351
publisher
Nature Publishing Group
external identifiers
  • wos:000282250400011
  • scopus:77956996314
ISSN
1751-7362
DOI
10.1038/ismej.2010.58
project
Microbial carbon-use efficiency
Effect of environmental factors on fungal and bacterial growth in soil
BECC
language
English
LU publication?
yes
id
3eef463f-e8b9-4220-a4ed-249e3f8fd262 (old id 1610571)
date added to LUP
2010-06-14 15:30:01
date last changed
2018-07-15 03:08:11
@article{3eef463f-e8b9-4220-a4ed-249e3f8fd262,
  abstract     = {Soils collected across a long-term liming experiment (pH 4.0-8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria. We hypothesized that bacterial communities would be more strongly influenced by pH than fungal communities. To determine the relative abundance of bacteria and fungi, we used quantitative PCR (qPCR), and to analyze the composition and diversity of the bacterial and fungal communities, we used a bar-coded pyrosequencing technique. Both the relative abundance and diversity of bacteria were positively related to pH, the latter nearly doubling between pH 4 and 8. In contrast, the relative abundance of fungi was unaffected by pH and fungal diversity was only weakly related with pH. The composition of the bacterial communities was closely defined by soil pH; there was as much variability in bacterial community composition across the 180-m distance of this liming experiment as across soils collected from a wide range of biomes in North and South America, emphasizing the dominance of pH in structuring bacterial communities. The apparent direct influence of pH on bacterial community composition is probably due to the narrow pH ranges for optimal growth of bacteria. Fungal community composition was less strongly affected by pH, which is consistent with pure culture studies, demonstrating that fungi generally exhibit wider pH ranges for optimal growth.},
  author       = {Rousk, Johannes and Bååth, Erland and Brookes, Philip C and Lauber, Christian L and Lozupone, Catherine and Caporaso, J Gregory and Knight, Rob and Fierer, Noah},
  issn         = {1751-7362},
  language     = {eng},
  pages        = {1340--1351},
  publisher    = {Nature Publishing Group},
  series       = {The Isme Journal},
  title        = {Soil bacterial and fungal communities across a pH gradient in an arable soil.},
  url          = {http://dx.doi.org/10.1038/ismej.2010.58},
  volume       = {4},
  year         = {2010},
}