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Cryptic terminal chromosome rearrangements in colorectal carcinoma cell lines detected by subtelomeric FISH analysis.

Stewénius, Ylva LU ; Tanke, H J ; Wiegant, J and Gisselsson Nord, David LU (2006) In Cytogenetic and Genome Research 114(3-4). p.257-262
Abstract
Epithelial tumour karyotypes are often difficult to study by standard cytogenetic methods because of poor chromosome preparation quality and the high complexity of their genomic rearrangements. Subtelomeric fluorescence in situ hybridisation (FISH) has proved to be a useful method for detecting cryptic constitutional chromosomal rearrangements but little is known about its usefulness for tumour cytogenetic analysis. Using a combination of chromosome banding, multicolour karyotyping and subtelomeric FISH, five colorectal cancer cell lines were characterised. The resulting data were compared to results from previous studies by comparative genomic hybridisation and spectral karyotyping or multicolour FISH. Subtelomeric FISH made it possible... (More)
Epithelial tumour karyotypes are often difficult to study by standard cytogenetic methods because of poor chromosome preparation quality and the high complexity of their genomic rearrangements. Subtelomeric fluorescence in situ hybridisation (FISH) has proved to be a useful method for detecting cryptic constitutional chromosomal rearrangements but little is known about its usefulness for tumour cytogenetic analysis. Using a combination of chromosome banding, multicolour karyotyping and subtelomeric FISH, five colorectal cancer cell lines were characterised. The resulting data were compared to results from previous studies by comparative genomic hybridisation and spectral karyotyping or multicolour FISH. Subtelomeric FISH made it possible to resolve several highly complex chromosome rearrangements, many of which had not been detected or were incompletely characterised by the other methods. In particular, previously undetected terminal imbalances were found in the two cell lines not showing microsatellite instability. Copyright (c) 2006 S. Karger AG, Basel (Less)
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type
Contribution to journal
publication status
published
subject
in
Cytogenetic and Genome Research
volume
114
issue
3-4
pages
257 - 262
publisher
Karger
external identifiers
  • pmid:16954663
  • wos:000240328000010
  • scopus:33748449472
ISSN
1424-859X
DOI
10.1159/000094210
language
English
LU publication?
yes
id
36a25607-af18-42e6-b31e-5b7e6dbbc1d0 (old id 161203)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16954663&dopt=Abstract
date added to LUP
2016-04-01 11:58:58
date last changed
2021-06-30 05:34:29
@article{36a25607-af18-42e6-b31e-5b7e6dbbc1d0,
  abstract     = {Epithelial tumour karyotypes are often difficult to study by standard cytogenetic methods because of poor chromosome preparation quality and the high complexity of their genomic rearrangements. Subtelomeric fluorescence in situ hybridisation (FISH) has proved to be a useful method for detecting cryptic constitutional chromosomal rearrangements but little is known about its usefulness for tumour cytogenetic analysis. Using a combination of chromosome banding, multicolour karyotyping and subtelomeric FISH, five colorectal cancer cell lines were characterised. The resulting data were compared to results from previous studies by comparative genomic hybridisation and spectral karyotyping or multicolour FISH. Subtelomeric FISH made it possible to resolve several highly complex chromosome rearrangements, many of which had not been detected or were incompletely characterised by the other methods. In particular, previously undetected terminal imbalances were found in the two cell lines not showing microsatellite instability. Copyright (c) 2006 S. Karger AG, Basel},
  author       = {Stewénius, Ylva and Tanke, H J and Wiegant, J and Gisselsson Nord, David},
  issn         = {1424-859X},
  language     = {eng},
  number       = {3-4},
  pages        = {257--262},
  publisher    = {Karger},
  series       = {Cytogenetic and Genome Research},
  title        = {Cryptic terminal chromosome rearrangements in colorectal carcinoma cell lines detected by subtelomeric FISH analysis.},
  url          = {http://dx.doi.org/10.1159/000094210},
  doi          = {10.1159/000094210},
  volume       = {114},
  year         = {2006},
}