Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Prepatterning in the stem cell compartment

Tonge, Peter D. ; Olariu, Victor LU ; Coca, Daniel ; Kadirkamanathan, Visakan ; Burrell, Kelly E. ; Billings, Stephen A. and Andrews, Peter W. (2010) In PLoS ONE 5(5).
Abstract

The mechanism by which an apparently uniform population of cells can generate a heterogeneous population of differentiated derivatives is a fundamental aspect of pluripotent and multipotent stem cell behaviour. One possibility is that the environment and the differentiation cues to which the cells are exposed are not uniform. An alternative, but not mutually exclusive possibility is that the observed heterogeneity arises from the stem cells themselves through the existence of different interconvertible substates that pre-exist before the cells commit to differentiate. We have tested this hypothesis in the case of apparently homogeneous pluripotent human embryonal carcinoma (EC) stem cells, which do not follow a uniform pattern of... (More)

The mechanism by which an apparently uniform population of cells can generate a heterogeneous population of differentiated derivatives is a fundamental aspect of pluripotent and multipotent stem cell behaviour. One possibility is that the environment and the differentiation cues to which the cells are exposed are not uniform. An alternative, but not mutually exclusive possibility is that the observed heterogeneity arises from the stem cells themselves through the existence of different interconvertible substates that pre-exist before the cells commit to differentiate. We have tested this hypothesis in the case of apparently homogeneous pluripotent human embryonal carcinoma (EC) stem cells, which do not follow a uniform pattern of differentiation when exposed to retinoic acid. Instead, they produce differentiated progeny that include both neuronal and non-neural phenotypes. Our results suggest that pluripotent NTERA2 stem cells oscillate between functionally distinct substates that are primed to select distinct lineages when differentiation is induced.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
5
issue
5
article number
e10901
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:20531938
  • scopus:77956527493
ISSN
1932-6203
DOI
10.1371/journal.pone.0010901
language
English
LU publication?
no
id
1612a210-7f7b-46e3-9d48-cf0981e5b927
date added to LUP
2019-05-24 13:59:40
date last changed
2024-01-30 19:20:27
@article{1612a210-7f7b-46e3-9d48-cf0981e5b927,
  abstract     = {{<p>The mechanism by which an apparently uniform population of cells can generate a heterogeneous population of differentiated derivatives is a fundamental aspect of pluripotent and multipotent stem cell behaviour. One possibility is that the environment and the differentiation cues to which the cells are exposed are not uniform. An alternative, but not mutually exclusive possibility is that the observed heterogeneity arises from the stem cells themselves through the existence of different interconvertible substates that pre-exist before the cells commit to differentiate. We have tested this hypothesis in the case of apparently homogeneous pluripotent human embryonal carcinoma (EC) stem cells, which do not follow a uniform pattern of differentiation when exposed to retinoic acid. Instead, they produce differentiated progeny that include both neuronal and non-neural phenotypes. Our results suggest that pluripotent NTERA2 stem cells oscillate between functionally distinct substates that are primed to select distinct lineages when differentiation is induced.</p>}},
  author       = {{Tonge, Peter D. and Olariu, Victor and Coca, Daniel and Kadirkamanathan, Visakan and Burrell, Kelly E. and Billings, Stephen A. and Andrews, Peter W.}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{5}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Prepatterning in the stem cell compartment}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0010901}},
  doi          = {{10.1371/journal.pone.0010901}},
  volume       = {{5}},
  year         = {{2010}},
}