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FTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin

Greve, Tanja M.; Andersen, Kristine B.; Nielsen, Ole F. and Engdahl, Anders LU (2010) 13th European Conference on Spectroscopy of Biological Mol In Spectroscopy: An International Journal 24(1-2). p.105-111
Abstract
FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C=O stretch and C-H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed hands in the region from 100-150 cm(-1) due to hydrogen bonded proteins and a band around 180 cm(-1) arising from "free" water.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
pig, ear skin, synchrotron radiation, FTIR imaging, ATR-FT-Far-IR spectroscopy
in
Spectroscopy: An International Journal
volume
24
issue
1-2
pages
105 - 111
publisher
IOS Press
conference name
13th European Conference on Spectroscopy of Biological Mol
external identifiers
  • wos:000278029800013
  • scopus:77953757705
ISSN
0712-4813
DOI
10.3233/SPE-2010-0413
language
English
LU publication?
yes
id
9fed2977-4fa7-4de7-a767-a0f8b934795a (old id 1616599)
date added to LUP
2010-06-22 15:46:34
date last changed
2018-05-29 09:29:09
@inproceedings{9fed2977-4fa7-4de7-a767-a0f8b934795a,
  abstract     = {FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C=O stretch and C-H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed hands in the region from 100-150 cm(-1) due to hydrogen bonded proteins and a band around 180 cm(-1) arising from "free" water.},
  author       = {Greve, Tanja M. and Andersen, Kristine B. and Nielsen, Ole F. and Engdahl, Anders},
  booktitle    = {Spectroscopy: An International Journal},
  issn         = {0712-4813},
  keyword      = {pig,ear skin,synchrotron radiation,FTIR imaging,ATR-FT-Far-IR spectroscopy},
  language     = {eng},
  number       = {1-2},
  pages        = {105--111},
  publisher    = {IOS Press},
  title        = {FTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin},
  url          = {http://dx.doi.org/10.3233/SPE-2010-0413},
  volume       = {24},
  year         = {2010},
}