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Calcitonin gene-related peptide (CGRP) levels during glyceryl trinitrate (GTN)-induced headache in healthy volunteers

Kruuse, C.; Iversen, H. K.; Jansen-Olesen, I.; Edvinsson, Lars LU and Olesen, J. (2010) In Cephalalgia 30(4). p.467-474
Abstract
The role of nitric oxide (NO) in migraine has been studied in the experimental glyceryl trinitrate (GTN)-infusion headache model. We hypothesized that GTN-induced headache may activate the trigeminovascular system and be associated with increased levels of sensory neuropeptides, including calcitonin gene-related peptide (CGRP). CGRP, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY) and somatostatin plasma levels were measured before and after placebo/sumatriptan injection and during GTN-induced headache. Following a double-blind randomized cross-over design, 10 healthy volunteers received subcutaneous sumatriptan 6 mg or placebo. This was succeeded by 20 min of GTN (0.12 mu g kg(-1) min(-1)) infusion. At baseline no subject... (More)
The role of nitric oxide (NO) in migraine has been studied in the experimental glyceryl trinitrate (GTN)-infusion headache model. We hypothesized that GTN-induced headache may activate the trigeminovascular system and be associated with increased levels of sensory neuropeptides, including calcitonin gene-related peptide (CGRP). CGRP, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY) and somatostatin plasma levels were measured before and after placebo/sumatriptan injection and during GTN-induced headache. Following a double-blind randomized cross-over design, 10 healthy volunteers received subcutaneous sumatriptan 6 mg or placebo. This was succeeded by 20 min of GTN (0.12 mu g kg(-1) min(-1)) infusion. At baseline no subject reported headache (using verbal rating scale from 0 to 10) and the jugular CGRP-like immunoreactivity (-LI) level was 18.6 +/- 2.5 pmol/l. After a 20-min intravenous infusion of GTN 0.12 mu g kg(-1) min(-1), median peak headache intensity was 4 (range 2-6) (P < 0.05), while jugular CGRP-LI levels were unchanged (19.0 +/- 2.8 pmol/l; P > 0.05). There were no changes in VIP-, NPY- or somatostatin-LI. In conclusion, the NO donor GTN appears not to induce headache via immediate CGRP release. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
headache, Nitric oxide, CGRP, plasma, sumatriptan
in
Cephalalgia
volume
30
issue
4
pages
467 - 474
publisher
Wiley-Blackwell
external identifiers
  • wos:000277586100010
  • scopus:77956247519
ISSN
0333-1024
DOI
10.1111/j.1468-2982.2009.01963.x
language
English
LU publication?
yes
id
04203529-21a5-45dd-8449-01ef9283f5ed (old id 1618021)
date added to LUP
2010-06-23 13:19:48
date last changed
2018-06-10 04:16:36
@article{04203529-21a5-45dd-8449-01ef9283f5ed,
  abstract     = {The role of nitric oxide (NO) in migraine has been studied in the experimental glyceryl trinitrate (GTN)-infusion headache model. We hypothesized that GTN-induced headache may activate the trigeminovascular system and be associated with increased levels of sensory neuropeptides, including calcitonin gene-related peptide (CGRP). CGRP, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY) and somatostatin plasma levels were measured before and after placebo/sumatriptan injection and during GTN-induced headache. Following a double-blind randomized cross-over design, 10 healthy volunteers received subcutaneous sumatriptan 6 mg or placebo. This was succeeded by 20 min of GTN (0.12 mu g kg(-1) min(-1)) infusion. At baseline no subject reported headache (using verbal rating scale from 0 to 10) and the jugular CGRP-like immunoreactivity (-LI) level was 18.6 +/- 2.5 pmol/l. After a 20-min intravenous infusion of GTN 0.12 mu g kg(-1) min(-1), median peak headache intensity was 4 (range 2-6) (P &lt; 0.05), while jugular CGRP-LI levels were unchanged (19.0 +/- 2.8 pmol/l; P &gt; 0.05). There were no changes in VIP-, NPY- or somatostatin-LI. In conclusion, the NO donor GTN appears not to induce headache via immediate CGRP release.},
  author       = {Kruuse, C. and Iversen, H. K. and Jansen-Olesen, I. and Edvinsson, Lars and Olesen, J.},
  issn         = {0333-1024},
  keyword      = {headache,Nitric oxide,CGRP,plasma,sumatriptan},
  language     = {eng},
  number       = {4},
  pages        = {467--474},
  publisher    = {Wiley-Blackwell},
  series       = {Cephalalgia},
  title        = {Calcitonin gene-related peptide (CGRP) levels during glyceryl trinitrate (GTN)-induced headache in healthy volunteers},
  url          = {http://dx.doi.org/10.1111/j.1468-2982.2009.01963.x},
  volume       = {30},
  year         = {2010},
}