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Pyrophosphate as a central energy carrier in the hydrogen-producing extremely thermophilic Caldicellulosiruptor saccharolyticus

Bielen, Abraham A. M. ; Willquist, Karin LU ; Engman, Jakob LU ; van der Oost, John ; van Niel, Ed LU and Kengen, Serve W. M. (2010) In FEMS Microbiology Letters 307(1). p.48-54
Abstract
The role of inorganic pyrophosphate (PPi) as an energy carrier in the central metabolism of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus was investigated. In agreement with its annotated genome sequence, cell extracts were shown to exhibit PPi-dependent phosphofructokinase and pyruvate phosphate dikinase activity. In addition, membrane-bound pyrophosphatase activity was demonstrated, while no significant cytosolic pyrophosphatase activity was detected. During the exponential growth phase, high PPi levels (approximately 4 +/- 2 mM) and relatively low ATP levels (0.43 +/- 0.07 mM) were found, and the PPi/ATP ratio decreased 13-fold when the cells entered the stationary phase. Pyruvate kinase activity appeared to... (More)
The role of inorganic pyrophosphate (PPi) as an energy carrier in the central metabolism of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus was investigated. In agreement with its annotated genome sequence, cell extracts were shown to exhibit PPi-dependent phosphofructokinase and pyruvate phosphate dikinase activity. In addition, membrane-bound pyrophosphatase activity was demonstrated, while no significant cytosolic pyrophosphatase activity was detected. During the exponential growth phase, high PPi levels (approximately 4 +/- 2 mM) and relatively low ATP levels (0.43 +/- 0.07 mM) were found, and the PPi/ATP ratio decreased 13-fold when the cells entered the stationary phase. Pyruvate kinase activity appeared to be allosterically affected by PPi. Altogether, these findings suggest an important role for PPi in the central energy metabolism of C. saccharolyticus. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phosphofructokinase, pyrophosphate, H plus -PPase, Caldicellulosiruptor saccharolyticus, glycolysis
in
FEMS Microbiology Letters
volume
307
issue
1
pages
48 - 54
publisher
Oxford University Press
external identifiers
  • wos:000277321800008
  • scopus:77951942319
  • pmid:20557574
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2010.01957.x
language
English
LU publication?
yes
id
e17b1989-d474-46fd-9b28-6c7138677514 (old id 1618603)
date added to LUP
2016-04-01 13:36:49
date last changed
2022-03-21 19:30:31
@article{e17b1989-d474-46fd-9b28-6c7138677514,
  abstract     = {{The role of inorganic pyrophosphate (PPi) as an energy carrier in the central metabolism of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus was investigated. In agreement with its annotated genome sequence, cell extracts were shown to exhibit PPi-dependent phosphofructokinase and pyruvate phosphate dikinase activity. In addition, membrane-bound pyrophosphatase activity was demonstrated, while no significant cytosolic pyrophosphatase activity was detected. During the exponential growth phase, high PPi levels (approximately 4 +/- 2 mM) and relatively low ATP levels (0.43 +/- 0.07 mM) were found, and the PPi/ATP ratio decreased 13-fold when the cells entered the stationary phase. Pyruvate kinase activity appeared to be allosterically affected by PPi. Altogether, these findings suggest an important role for PPi in the central energy metabolism of C. saccharolyticus.}},
  author       = {{Bielen, Abraham A. M. and Willquist, Karin and Engman, Jakob and van der Oost, John and van Niel, Ed and Kengen, Serve W. M.}},
  issn         = {{1574-6968}},
  keywords     = {{phosphofructokinase; pyrophosphate; H plus -PPase; Caldicellulosiruptor saccharolyticus; glycolysis}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{48--54}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{Pyrophosphate as a central energy carrier in the hydrogen-producing extremely thermophilic Caldicellulosiruptor saccharolyticus}},
  url          = {{https://lup.lub.lu.se/search/files/3477850/1760906.pdf}},
  doi          = {{10.1111/j.1574-6968.2010.01957.x}},
  volume       = {{307}},
  year         = {{2010}},
}