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Maternal plasma level of antimicrobial peptide LL37 is a major determinant factor of neonatal plasma LL37 level

Havelka, A. Mandic; Yektaei-Karin, E.; Hultenby, K.; Sørensen, Ole E LU ; Lundahl, J.; Berggren, V. and Marchini, G. (2010) In Acta Pædiatrica 99(6). p.836-841
Abstract
Aim: To determine cathelicidin antimicrobial peptide LL37subcellular distribution in cord neutrophils and normal plasma LL37 levels in mothers and neonates, relate them to delivery mode and relevant biochemical markers, including 25-OHvitamin D [25(OH)D] as this molecules increases cathelicidin gene expression. Methods: A total of 115 infants were included, n = 68 with normal delivery and n = 47 with elective Caesarean section (C-section), a subset of these being 50 mother-infant pairs. Biomarkers were determined in maternal and cord blood. Subcellular peptide LL37 distribution was analysed with immunoelectron microscopy. Results: Cord plasma LL37 levels were three-times higher after normal delivery compared with C-section. A highly... (More)
Aim: To determine cathelicidin antimicrobial peptide LL37subcellular distribution in cord neutrophils and normal plasma LL37 levels in mothers and neonates, relate them to delivery mode and relevant biochemical markers, including 25-OHvitamin D [25(OH)D] as this molecules increases cathelicidin gene expression. Methods: A total of 115 infants were included, n = 68 with normal delivery and n = 47 with elective Caesarean section (C-section), a subset of these being 50 mother-infant pairs. Biomarkers were determined in maternal and cord blood. Subcellular peptide LL37 distribution was analysed with immunoelectron microscopy. Results: Cord plasma LL37 levels were three-times higher after normal delivery compared with C-section. A highly significant correlation was observed between maternal and cord plasma LL37 levels, regardless of delivery mode. No relationship was found between LL37 and 25(OH)D levels. Neutrophils from cord blood after normal delivery contained 10-times more cytoplasmatic cathelicidin peptide compared with corresponding cells after C-section where a strict granular localization was found. Conclusion: These data are consistent with a placental transfer of LL37 and identifies maternal stores as the critical factor determining neonatal plasma LL37 level. An additional enhancement of neonatal cathelicidin mobilization and release is connected to normal delivery stress. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Newborn infant, Mode of delivery, Maternal immunity, Cathelicidin, Innate immunity
in
Acta Pædiatrica
volume
99
issue
6
pages
836 - 841
publisher
Wiley-Blackwell Publishing Ltd
external identifiers
  • wos:000277320300012
  • scopus:77951946876
ISSN
1651-2227
DOI
10.1111/j.1651-2227.2010.01726.x
language
English
LU publication?
yes
id
78990545-6d0f-4964-a866-81f69a667b66 (old id 1618984)
date added to LUP
2010-06-18 13:28:44
date last changed
2018-07-01 04:03:12
@article{78990545-6d0f-4964-a866-81f69a667b66,
  abstract     = {Aim: To determine cathelicidin antimicrobial peptide LL37subcellular distribution in cord neutrophils and normal plasma LL37 levels in mothers and neonates, relate them to delivery mode and relevant biochemical markers, including 25-OHvitamin D [25(OH)D] as this molecules increases cathelicidin gene expression. Methods: A total of 115 infants were included, n = 68 with normal delivery and n = 47 with elective Caesarean section (C-section), a subset of these being 50 mother-infant pairs. Biomarkers were determined in maternal and cord blood. Subcellular peptide LL37 distribution was analysed with immunoelectron microscopy. Results: Cord plasma LL37 levels were three-times higher after normal delivery compared with C-section. A highly significant correlation was observed between maternal and cord plasma LL37 levels, regardless of delivery mode. No relationship was found between LL37 and 25(OH)D levels. Neutrophils from cord blood after normal delivery contained 10-times more cytoplasmatic cathelicidin peptide compared with corresponding cells after C-section where a strict granular localization was found. Conclusion: These data are consistent with a placental transfer of LL37 and identifies maternal stores as the critical factor determining neonatal plasma LL37 level. An additional enhancement of neonatal cathelicidin mobilization and release is connected to normal delivery stress.},
  author       = {Havelka, A. Mandic and Yektaei-Karin, E. and Hultenby, K. and Sørensen, Ole E and Lundahl, J. and Berggren, V. and Marchini, G.},
  issn         = {1651-2227},
  keyword      = {Newborn infant,Mode of delivery,Maternal immunity,Cathelicidin,Innate immunity},
  language     = {eng},
  number       = {6},
  pages        = {836--841},
  publisher    = {Wiley-Blackwell Publishing Ltd},
  series       = {Acta Pædiatrica},
  title        = {Maternal plasma level of antimicrobial peptide LL37 is a major determinant factor of neonatal plasma LL37 level},
  url          = {http://dx.doi.org/10.1111/j.1651-2227.2010.01726.x},
  volume       = {99},
  year         = {2010},
}