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Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy

Spadiut, Oliver; Brugger, Dagmar; Coman, Vasile LU ; Haltrich, Dietmar and Gorton, Lo LU (2010) In Electroanalysis 22(7-8). p.813-820
Abstract
Due to the recent interest in enzymatic biofuel cells (BECs), sugar oxidizing enzymes other than the commonly used glucose oxidase are gaining more importance as possible bioelements of implantable microscale-devices, which can, for example, be used in biosensors and pacemakers. In this study we used rational and semi-rational protein design to improve the catalytic activity of the enzyme pyranose 2-oxidase (P2Ox) with its alternative soluble electron acceptors 1,4-benzoquinone and ferricenium ion, which can serve as electron mediators, to possibly boost the power output of enzymatic BECs. Using a screening assay based on 96-well plates, we identified the variant H450G, which showed lower K-M and higher k(cat) values for both... (More)
Due to the recent interest in enzymatic biofuel cells (BECs), sugar oxidizing enzymes other than the commonly used glucose oxidase are gaining more importance as possible bioelements of implantable microscale-devices, which can, for example, be used in biosensors and pacemakers. In this study we used rational and semi-rational protein design to improve the catalytic activity of the enzyme pyranose 2-oxidase (P2Ox) with its alternative soluble electron acceptors 1,4-benzoquinone and ferricenium ion, which can serve as electron mediators, to possibly boost the power output of enzymatic BECs. Using a screening assay based on 96-well plates, we identified the variant H450G, which showed lower K-M and higher k(cat) values for both 1,4-benzoquinone and ferricenium ion compared to the wild-type enzyme, when either D-glucose or D-galactose were used as saturating electron donors. Besides this variant, we analyzed the variants V546C and T169G/V546C for their possible application in enzymatic BECs. The results obtained in homogeneous solution were compared with those obtained when P2Ox was immobilized on the surface of graphite electrodes and either "wired" to an osmium redox polymer or using soluble 1,4-benzoquinone as mediator. According to the spectrophotometrically determined kinetic constants, the possible energy output, measured in flow injection analysis experiments with these variants, increased up to 4-fold compared to systems employing the wild-type enzyme. Our results show that by increasing the catalytic activity of the redox enzyme P2Ox with its alternative electron acceptors 1,4-benzoquinone and ferricenium ion, it is possible to achieve a higher energy output of an enzymatic BFC when using the same concentration of sugar substrate. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
4-Benzoquinone, 1, polymer, Osmium redox, Mediated electron transfer, Enzymatic biofuel cell, Rational protein design, Pyranose 2-oxidase, Sugar oxidoreductases, Fuel cells
in
Electroanalysis
volume
22
issue
7-8
pages
813 - 820
publisher
John Wiley & Sons
external identifiers
  • wos:000277255400012
  • scopus:77950996000
ISSN
1040-0397
DOI
10.1002/elan.200980015
language
English
LU publication?
yes
id
fcc0de09-2b47-45ab-9e87-efcce720971c (old id 1619483)
date added to LUP
2010-06-18 12:03:15
date last changed
2018-07-22 03:37:21
@article{fcc0de09-2b47-45ab-9e87-efcce720971c,
  abstract     = {Due to the recent interest in enzymatic biofuel cells (BECs), sugar oxidizing enzymes other than the commonly used glucose oxidase are gaining more importance as possible bioelements of implantable microscale-devices, which can, for example, be used in biosensors and pacemakers. In this study we used rational and semi-rational protein design to improve the catalytic activity of the enzyme pyranose 2-oxidase (P2Ox) with its alternative soluble electron acceptors 1,4-benzoquinone and ferricenium ion, which can serve as electron mediators, to possibly boost the power output of enzymatic BECs. Using a screening assay based on 96-well plates, we identified the variant H450G, which showed lower K-M and higher k(cat) values for both 1,4-benzoquinone and ferricenium ion compared to the wild-type enzyme, when either D-glucose or D-galactose were used as saturating electron donors. Besides this variant, we analyzed the variants V546C and T169G/V546C for their possible application in enzymatic BECs. The results obtained in homogeneous solution were compared with those obtained when P2Ox was immobilized on the surface of graphite electrodes and either "wired" to an osmium redox polymer or using soluble 1,4-benzoquinone as mediator. According to the spectrophotometrically determined kinetic constants, the possible energy output, measured in flow injection analysis experiments with these variants, increased up to 4-fold compared to systems employing the wild-type enzyme. Our results show that by increasing the catalytic activity of the redox enzyme P2Ox with its alternative electron acceptors 1,4-benzoquinone and ferricenium ion, it is possible to achieve a higher energy output of an enzymatic BFC when using the same concentration of sugar substrate.},
  author       = {Spadiut, Oliver and Brugger, Dagmar and Coman, Vasile and Haltrich, Dietmar and Gorton, Lo},
  issn         = {1040-0397},
  keyword      = {4-Benzoquinone,1,polymer,Osmium redox,Mediated electron transfer,Enzymatic biofuel cell,Rational protein design,Pyranose 2-oxidase,Sugar oxidoreductases,Fuel cells},
  language     = {eng},
  number       = {7-8},
  pages        = {813--820},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy},
  url          = {http://dx.doi.org/10.1002/elan.200980015},
  volume       = {22},
  year         = {2010},
}