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Molecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinoma.

Jin, Charlotte LU ; Jin, Yuesheng LU ; Gisselsson Nord, David LU ; Wennerberg, Johan LU orcid ; Wah, T S ; Stromback, B ; Kwong, Y-L and Mertens, Fredrik LU (2006) In Cytogenetic and Genome Research 115(2). p.99-106
Abstract
Amplification of 11q13 DNA sequences and overexpression of CCND1 are common findings in head and neck squamous cell carcinoma (HNSCC), identified in about 30% of the cases. However, little is known about initiation of the amplification and the organization of the amplicon. In order to study the structure of the amplicon in more detail and to learn more about the mechanisms involved in its initiation, prometaphase, metaphase, and anaphase fluorescence in situ hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with homogeneously staining regions. FISH analysis showed that the size of the amplicon varied among the nine cell lines, the smallest being... (More)
Amplification of 11q13 DNA sequences and overexpression of CCND1 are common findings in head and neck squamous cell carcinoma (HNSCC), identified in about 30% of the cases. However, little is known about initiation of the amplification and the organization of the amplicon. In order to study the structure of the amplicon in more detail and to learn more about the mechanisms involved in its initiation, prometaphase, metaphase, and anaphase fluorescence in situ hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with homogeneously staining regions. FISH analysis showed that the size of the amplicon varied among the nine cell lines, the smallest being 2.12 Mb and the largest 8.97 Mb. The smallest overlapping region of amplification was approximately 1.61 Mb, covering the region from BAC 729E14 to BAC 102B19. This region contained several genes previously shown to be amplified and overexpressed in HNSCC, including CCDN1, CTTN, SHANK2, and ORAOV1. The cell lines were also used to study the internal structure of the amplicon. Various patterns of amplified DNA sequences within the amplicon were found among the nine cell lines. Even within the same cell line, different amplicon structures could be found in different cell populations, indicating that the mechanisms involved in the development of the amplicons in HNSCC were more complex than previously assumed. The frequent finding of inverted repeats within the amplicons, however, suggests that breakage-fusion-bridge cycles are important in the initiation, but the fact that such repeats constituted only small parts of the amplicons indicate that they are further rearranged during tumor progression. Copyright (c) 2006 S. Karger AG, Basel. (Less)
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type
Contribution to journal
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published
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in
Cytogenetic and Genome Research
volume
115
issue
2
pages
99 - 106
publisher
Karger
external identifiers
  • wos:000241664700001
  • scopus:33750362122
ISSN
1424-859X
DOI
10.1159/000095228
language
English
LU publication?
yes
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f4ae0c67-052d-4b24-8699-61d1a5e42c91 (old id 162137)
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17065789&dopt=Abstract
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2016-04-01 11:51:21
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2021-09-29 01:24:31
@article{f4ae0c67-052d-4b24-8699-61d1a5e42c91,
  abstract     = {Amplification of 11q13 DNA sequences and overexpression of CCND1 are common findings in head and neck squamous cell carcinoma (HNSCC), identified in about 30% of the cases. However, little is known about initiation of the amplification and the organization of the amplicon. In order to study the structure of the amplicon in more detail and to learn more about the mechanisms involved in its initiation, prometaphase, metaphase, and anaphase fluorescence in situ hybridization (FISH) with 40 BAC clones spanning a 16-Mb region in chromosome bands 11q12.2 to 11q13.5 was performed in nine HNSCC cell lines with homogeneously staining regions. FISH analysis showed that the size of the amplicon varied among the nine cell lines, the smallest being 2.12 Mb and the largest 8.97 Mb. The smallest overlapping region of amplification was approximately 1.61 Mb, covering the region from BAC 729E14 to BAC 102B19. This region contained several genes previously shown to be amplified and overexpressed in HNSCC, including CCDN1, CTTN, SHANK2, and ORAOV1. The cell lines were also used to study the internal structure of the amplicon. Various patterns of amplified DNA sequences within the amplicon were found among the nine cell lines. Even within the same cell line, different amplicon structures could be found in different cell populations, indicating that the mechanisms involved in the development of the amplicons in HNSCC were more complex than previously assumed. The frequent finding of inverted repeats within the amplicons, however, suggests that breakage-fusion-bridge cycles are important in the initiation, but the fact that such repeats constituted only small parts of the amplicons indicate that they are further rearranged during tumor progression. Copyright (c) 2006 S. Karger AG, Basel.},
  author       = {Jin, Charlotte and Jin, Yuesheng and Gisselsson Nord, David and Wennerberg, Johan and Wah, T S and Stromback, B and Kwong, Y-L and Mertens, Fredrik},
  issn         = {1424-859X},
  language     = {eng},
  number       = {2},
  pages        = {99--106},
  publisher    = {Karger},
  series       = {Cytogenetic and Genome Research},
  title        = {Molecular cytogenetic characterization of the 11q13 amplicon in head and neck squamous cell carcinoma.},
  url          = {http://dx.doi.org/10.1159/000095228},
  doi          = {10.1159/000095228},
  volume       = {115},
  year         = {2006},
}