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A critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade.

Jimenez, Maria A; Akerblad, Peter; Sigvardsson, Mikael LU and Rosen, Evan D (2007) In Molecular and Cellular Biology 27(Oct 23). p.743-757
Abstract
The Ebf (O/E) family of helix-loop-helix transcription factors plays a significant role in B lymphocyte and neuronal development. The three primary members of this family, Ebf1, 2, and 3, are all expressed in adipocytes, and Ebf1 promotes adipogenesis when overexpressed in NIH 3T3 fibroblasts. Here we report that these three proteins have adipogenic potential in multiple cellular models and that peroxisome proliferator-activated receptor gamma (PPAR gamma) is required for this effect, at least in part due to direct activation of the PPAR gamma 1 promoter by Ebf1. Ebf1 also directly binds to and activates the C/EBP alpha promoter, which exerts positive feedback on C/EBP delta expression. Despite this, C/EBP alpha is dispensable for the... (More)
The Ebf (O/E) family of helix-loop-helix transcription factors plays a significant role in B lymphocyte and neuronal development. The three primary members of this family, Ebf1, 2, and 3, are all expressed in adipocytes, and Ebf1 promotes adipogenesis when overexpressed in NIH 3T3 fibroblasts. Here we report that these three proteins have adipogenic potential in multiple cellular models and that peroxisome proliferator-activated receptor gamma (PPAR gamma) is required for this effect, at least in part due to direct activation of the PPAR gamma 1 promoter by Ebf1. Ebf1 also directly binds to and activates the C/EBP alpha promoter, which exerts positive feedback on C/EBP delta expression. Despite this, C/EBP alpha is dispensable for the adipogenic action of Ebf proteins. Ebf1 itself is induced by C/EBP beta and delta, which bind and activate its promoter. Reduction of Ebf1 and Ebf2 proteins by specific short hairpin RNA blocks differentiation of 3T3-L1 cells, suggesting a critical role for these factors and the absence of functional redundancy between members of this family. Altogether, these data place Ebf1 within the known transcriptional cascade of adipogenesis and suggest critical roles for Ebf1 and Ebf2. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Biology
volume
27
issue
Oct 23
pages
743 - 757
publisher
American Society for Microbiology
external identifiers
  • wos:000243353500027
  • scopus:33846152299
ISSN
0270-7306
DOI
10.1128/MCB.01557-06
language
English
LU publication?
yes
id
57e49e62-182b-41f2-bcd9-1011106e382c (old id 162172)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17060461&dopt=Abstract
date added to LUP
2007-07-23 16:44:04
date last changed
2017-10-22 03:46:01
@article{57e49e62-182b-41f2-bcd9-1011106e382c,
  abstract     = {The Ebf (O/E) family of helix-loop-helix transcription factors plays a significant role in B lymphocyte and neuronal development. The three primary members of this family, Ebf1, 2, and 3, are all expressed in adipocytes, and Ebf1 promotes adipogenesis when overexpressed in NIH 3T3 fibroblasts. Here we report that these three proteins have adipogenic potential in multiple cellular models and that peroxisome proliferator-activated receptor gamma (PPAR gamma) is required for this effect, at least in part due to direct activation of the PPAR gamma 1 promoter by Ebf1. Ebf1 also directly binds to and activates the C/EBP alpha promoter, which exerts positive feedback on C/EBP delta expression. Despite this, C/EBP alpha is dispensable for the adipogenic action of Ebf proteins. Ebf1 itself is induced by C/EBP beta and delta, which bind and activate its promoter. Reduction of Ebf1 and Ebf2 proteins by specific short hairpin RNA blocks differentiation of 3T3-L1 cells, suggesting a critical role for these factors and the absence of functional redundancy between members of this family. Altogether, these data place Ebf1 within the known transcriptional cascade of adipogenesis and suggest critical roles for Ebf1 and Ebf2.},
  author       = {Jimenez, Maria A and Akerblad, Peter and Sigvardsson, Mikael and Rosen, Evan D},
  issn         = {0270-7306},
  language     = {eng},
  number       = {Oct 23},
  pages        = {743--757},
  publisher    = {American Society for Microbiology},
  series       = {Molecular and Cellular Biology},
  title        = {A critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade.},
  url          = {http://dx.doi.org/10.1128/MCB.01557-06},
  volume       = {27},
  year         = {2007},
}