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Expression pattern of JunD after acute or chronic l-DOPA treatment: Comparison with DeltaFosB.

Valastro, Barbara LU ; Andersson, M; Lindgren, Hanna LU and Cenci Nilsson, Angela LU (2007) In Neuroscience 144(Oct 19). p.198-207
Abstract
In this study, we have used 6-hydroxydopamine-lesioned rats to examine changes in striatal junD and fosB/Delta fosB expression induced by acute and chronic treatment with (L)-DOPA (5 and 15 days). Changes at the protein levels were studied using Western immunoblotting while mRNA changes were compared using in situ hybridization histochemistry. We observed a significant increase in the level of Delta FosB proteins after chronic treatment with L-DOPA, an effect that was not observed for JunD proteins. In addition, the upregulation of Delta FosB was already present after an acute treatment but increased upon chronic treatment. By contrast, junD and Delta fosB mRNA were both upregulated significantly above control levels after an acute... (More)
In this study, we have used 6-hydroxydopamine-lesioned rats to examine changes in striatal junD and fosB/Delta fosB expression induced by acute and chronic treatment with (L)-DOPA (5 and 15 days). Changes at the protein levels were studied using Western immunoblotting while mRNA changes were compared using in situ hybridization histochemistry. We observed a significant increase in the level of Delta FosB proteins after chronic treatment with L-DOPA, an effect that was not observed for JunD proteins. In addition, the upregulation of Delta FosB was already present after an acute treatment but increased upon chronic treatment. By contrast, junD and Delta fosB mRNA were both upregulated significantly above control levels after an acute injection of L-DOPA. In conclusion, this study suggests a differential expression pattern of junD and Delta fosB in a rat model of L-DOPA-induced dyskinesia. The upregulation of Delta FosB protein, but not JunD, is likely to reflect an increased stability of the Delta FosB proteins without ongoing enhanced transcription of the encoding genes. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transcription, immediate-early genes, striatum, factor AP1, dyskinesia, DOPA, Parkinson's disease
in
Neuroscience
volume
144
issue
Oct 19
pages
198 - 207
publisher
Elsevier
external identifiers
  • wos:000242860100020
  • scopus:33751571650
ISSN
1873-7544
DOI
10.1016/j.neuroscience.2006.09.001
language
English
LU publication?
yes
id
bc618c9d-297e-4802-aa48-3a1e22fb5ce8 (old id 162219)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17055656&dopt=Abstract
date added to LUP
2007-07-20 08:09:20
date last changed
2017-07-30 03:38:51
@article{bc618c9d-297e-4802-aa48-3a1e22fb5ce8,
  abstract     = {In this study, we have used 6-hydroxydopamine-lesioned rats to examine changes in striatal junD and fosB/Delta fosB expression induced by acute and chronic treatment with (L)-DOPA (5 and 15 days). Changes at the protein levels were studied using Western immunoblotting while mRNA changes were compared using in situ hybridization histochemistry. We observed a significant increase in the level of Delta FosB proteins after chronic treatment with L-DOPA, an effect that was not observed for JunD proteins. In addition, the upregulation of Delta FosB was already present after an acute treatment but increased upon chronic treatment. By contrast, junD and Delta fosB mRNA were both upregulated significantly above control levels after an acute injection of L-DOPA. In conclusion, this study suggests a differential expression pattern of junD and Delta fosB in a rat model of L-DOPA-induced dyskinesia. The upregulation of Delta FosB protein, but not JunD, is likely to reflect an increased stability of the Delta FosB proteins without ongoing enhanced transcription of the encoding genes. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.},
  author       = {Valastro, Barbara and Andersson, M and Lindgren, Hanna and Cenci Nilsson, Angela},
  issn         = {1873-7544},
  keyword      = {transcription,immediate-early genes,striatum,factor AP1,dyskinesia,DOPA,Parkinson's disease},
  language     = {eng},
  number       = {Oct 19},
  pages        = {198--207},
  publisher    = {Elsevier},
  series       = {Neuroscience},
  title        = {Expression pattern of JunD after acute or chronic l-DOPA treatment: Comparison with DeltaFosB.},
  url          = {http://dx.doi.org/10.1016/j.neuroscience.2006.09.001},
  volume       = {144},
  year         = {2007},
}